Bovine ATP-binding cassette sub-family A member 13 (ABCA13) ELISA Kit | ABCA13 elisa kit

Bovine ATP-binding cassette sub-family A member 13 (ABCA13) ELISA Kit

Cat. Num. AAA7246334

• Reactivity: Bovine
• Synonyms: ATP-binding cassette sub-family A member 13 (ABCA13); Bovine ATP-binding cassette sub-family A member 13 (ABCA13) ELISA Kit; ABCA13 elisa kit

• Reactivity: Bovine
• Specificity: This assay has high sensitivity and excellent specificity for detection of ABCA13. No significant cross-reactivity or interference between ABCA13 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ABCA13 and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 1.0 pg/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ABCA13 elisa kit

Intended Uses: This ABCA13 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine ABCA13. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: ABCA13 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ABCA13 antibody and an ABCA13-HRP conjugate. The assay sample and buffer are incubated together with ABCA13-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ABCA13 concentration since ABCA13 from samples and ABCA13-HRP conjugate compete for the anti-ABCA13 antibody binding site. Since the number of sites is limited, as more sites are occupied by ABCA13 from the sample, fewer sites are left to bind ABCA13-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ABCA13 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ABCA13 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 597709765
• NCBI GeneID: 154664
• NCBI Accession #: NP_689914.3
• NCBI GenBank Nucleotide #: NM_152701.4
• Molecular Weight: 258,321 Da
• NCBI Official Full Name: ATP-binding cassette sub-family A member 13
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family A (ABC1), member 13
• NCBI Official Symbol: ABCA13
• NCBI Protein Information: ATP-binding cassette sub-family A member 13; ATP binding cassette transporter A13; ATP-binding cassette sub-family A member 13 variant 2; ATP-binding cassette sub-family A member 13 variant 3
• UniProt Protein Name: ATP-binding cassette sub-family A member 13
• Protein Family: ATP-binding cassette sub-family
• UniProt Gene Name: ABCA13
• UniProt Entry Name: ABCAD_HUMAN

NCBI Description

In human, the ATP-binding cassette (ABC) family of transmembrane transporters has at least 48 genes and 7 gene subfamilies. This gene is a member of ABC gene subfamily A (ABCA). Genes within the ABCA family typically encode several thousand amino acids. Like other ABC transmembrane transporter proteins, this protein has 12 or more transmembrane alpha-helix domains that likely arrange to form a single central chamber with multiple substrate binding sites. It is also predicted to have two large extracellular domains and two nucleotide binding domains as is typical for ABCA proteins. Alternative splice variants have been described but their biological validity has not been demonstrated.[provided by RefSeq, Mar 2009]

Uniprot Description

ABCA13: In human, the ATP-binding cassette (ABC) family of transmembrane transporters has at least 48 genes and 7 gene subfamilies. This gene is a member of ABC gene subfamily A (ABCA). Genes within the ABCA family typically encode several thousand amino acids. Like other ABC transmembrane transporter proteins, this protein has 12 or more transmembrane alpha-helix domains that likely arrange to form a single central chamber with multiple substrate binding sites. It is also predicted to have two large extracellular domains and two nucleotide binding domains as is typical for ABCA proteins. Alternative splice variants have been described but their biological validity has not been demonstrated.[provided by RefSeq, Mar 2009]

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7p12.3

Cellular Component: integral to membrane

Molecular Function: transporter activity; ATPase activity; ATP binding

Biological Process: transport; metabolic process