Rabbit anti-Human JIP3 Polyclonal Antibody | anti-JIP3 antibody

JIP3 Antibody

Cat. Num. AAA9409646

• Gene Names: MAPK8IP3; syd; JIP3; SYD2; JIP-3; JSAP1
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: JIP3; Polyclonal Antibody; JIP3 Antibody; C-jun-amino-terminal kinase interacting protein 3; JIP-3; JNK MAP kinase scaffold protein 3; JNK-interacting protein 3; anti-JIP3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total JIP3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 1330

• Applicable Applications for anti-JIP3 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500~1:3000; Immunofluorescence: 1:100~1:500
• Immunogen Type: Peptide
• Immunogen Description: Synthesized peptide derived from internal of human JIP3.
• Target Name: JIP3
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells, using JIP3 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cells, using JIP3 antibody.)

Related Product Information for anti-JIP3 antibody

The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins By similarity. UniProtKB Q9ESN9Hattori A., DNA Res. 7:357-366(2000).Martin J., Nature 432:988-994(2004).Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).

Product Categories/Family for anti-JIP3 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 94557295
• NCBI GeneID: 23162
• NCBI Accession #: NP_001035529.1
• NCBI GenBank Nucleotide #: NM_001040439.1
• UniProt Accession #: Q9UPT6; Q96RY4; Q9H4I4; Q9H7P1; Q9NUG0; A2A2B3; A7E2B3
• Molecular Weight: 29,052 Da
• NCBI Official Full Name: C-Jun-amino-terminal kinase-interacting protein 3 isoform 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 8 interacting protein 3
• NCBI Official Symbol: MAPK8IP3
• NCBI Official Synonym Symbols: syd; JIP3; SYD2; JIP-3; JSAP1
• NCBI Protein Information: C-Jun-amino-terminal kinase-interacting protein 3
• UniProt Protein Name: C-Jun-amino-terminal kinase-interacting protein 3
• Protein Family: Putative uncharacterized protein
• UniProt Gene Name: MAPK8IP3
• UniProt Synonym Gene Names: JIP3; KIAA1066; JIP-3; JNK-interacting protein 3
• UniProt Entry Name: JIP3_HUMAN

NCBI Description

The protein encoded by this gene shares similarity with the product of Drosophila syd gene, required for the functional interaction of kinesin I with axonal cargo. Studies of the similar gene in mouse suggested that this protein may interact with, and regulate the activity of numerous protein kinases of the JNK signaling pathway, and thus function as a scaffold protein in neuronal cells. The C. elegans counterpart of this gene is found to regulate synaptic vesicle transport possibly by integrating JNK signaling and kinesin-1 transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

JIP3: a member of the JNK-interacting protein group of scaffold proteins that selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Shares similarity with the product of Drosophila syd gene, required for the functional interaction of kinesin I with axonal cargo. Studies in the mouse suggested that this protein may interact with and regulate the activity of numerous protein kinases of the JNK signaling pathway. The C. elegans counterpart of this gene is found to regulate synaptic vesicle transport possibly by integrating JNK signaling and kinesin-1 transport. Several alternatively spliced transcript variants have been described, but the full-length nature of some of these variants has not been determined.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: Golgi membrane; smooth endoplasmic reticulum; cytoplasm; dendrite; axolemma

Molecular Function: JUN kinase binding; MAP-kinase scaffold activity; receptor signaling complex scaffold activity; mitogen-activated protein kinase kinase binding; kinesin binding; mitogen-activated protein kinase kinase kinase binding

Biological Process: vesicle-mediated transport; axon guidance; protein localization; in utero embryonic development; regulation of gene expression; forebrain development; respiratory gaseous exchange; positive regulation of neuron differentiation; alveolus development; regulation of JNK cascade; activation of JNK activity; post-embryonic development