Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat 6-phosphofructokinase, liver type (PFKL) Standard Curve.)
Rat 6-Phosphofructokinase, Liver Type (PFKL) ELISA Kit | PFKL elisa kit
Rat 6-Phosphofructokinase, Liver Type (PFKL) ELISA Kit
Gene Names
Pfkl; PFK-L; ATP-PFK
Reactivity
Rat
Synonyms
6-Phosphofructokinase; Liver Type (PFKL); Rat 6-Phosphofructokinase; Liver Type (PFKL) ELISA Kit; PFKL; DKFZp686G1648; DKFZp686L2097; FLJ30173; FLJ40909; PFK-B; 6-phosphofructokinase; liver type; liver phosphofructokinase; liver-type 1-phosphofructokinase; phosphofructo-1-kinase isozyme B; phosphofructoki; PFKL elisa kit
Reactivity
Rat
Specificity
Rat 6-phosphofructokinase, liver type (PFKL) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PFKL. No significant cross-reactivity or interference between Rat PFKL and analogues was observed.
Sequence Length
780
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat 6-phosphofructokinase, liver type (PFKL) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat 6-phosphofructokinase, liver type (PFKL) Standard Curve.)
Related Product Information for PFKL elisa kit
Description: This Rat 6-phosphofructokinase, liver type (PFKL) ELISA Kit employs a two-site sandwich ELISA to quantitate PFKL in samples. An antibody specific for PFKL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PFKL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PFKL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PFKL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The PFKL gene encodes the liver isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. Mammalian PFK is a tetramer made up of various combinations of 3 subunits: muscle (PFKM), liver (PFKL), and platelet (PFKP), the genes for which are located on chromosomes 12q13, 21q22, and 10p, respectively. The composition of the tetramers differs according to the tissue type. Muscle and liver PFK are a homotetramers of 4M and 4L subunits, respectively. Erythrocytes contain both L and M subunits, which randomly tetramerize to form M4, L4, M3L, M2L2, and ML3 hybrid forms of the holoenzyme.
Background: The PFKL gene encodes the liver isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. Mammalian PFK is a tetramer made up of various combinations of 3 subunits: muscle (PFKM), liver (PFKL), and platelet (PFKP), the genes for which are located on chromosomes 12q13, 21q22, and 10p, respectively. The composition of the tetramers differs according to the tissue type. Muscle and liver PFK are a homotetramers of 4M and 4L subunits, respectively. Erythrocytes contain both L and M subunits, which randomly tetramerize to form M4, L4, M3L, M2L2, and ML3 hybrid forms of the holoenzyme.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ATP-dependent 6-phosphofructokinase, liver type
NCBI Official Synonym Full Names
phosphofructokinase, liver type
NCBI Official Symbol
Pfkl
NCBI Official Synonym Symbols
PFK-L; ATP-PFK
NCBI Protein Information
ATP-dependent 6-phosphofructokinase, liver type
UniProt Protein Name
ATP-dependent 6-phosphofructokinase, liver type
Protein Family
UniProt Gene Name
Pfkl
UniProt Synonym Gene Names
Pfk-l; ATP-PFK; PFK-L
NCBI Description
catalyses the synthesis and degradation of Fru-2;6-P2; mediates switching mechanism in glycolysis/gluconeogenesis opposing fluxes [RGD, Feb 2006]
Uniprot Description
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Research Articles on PFKL
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Product Notes
The Rat PFKL pfkl (Catalog #AAA9712126) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712126 ELISA Kit recognizes Rat PFKL. It is sometimes possible for the material contained within the vial of "6-Phosphofructokinase, Liver Type (PFKL), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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