Rabbit PFKL Polyclonal Antibody | anti-PFKL antibody

PFKL (E724) Polyclonal Antibody

Cat. Num. AAA3001916

• Gene Names: PFKL; PFK-B; PFK-L; ATP-PFK
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PFKL; Polyclonal Antibody; PFKL (E724) Polyclonal Antibody; 6-phosphofructokinase; liver type; Phosphofructokinase 1; Phosphohexokinase; Phosphofructo-1-kinase isozyme B; PFK-B; anti-PFKL antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PFKL (E724) polyclonal antibody detects endogenous levels of PFKL protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 780

• Applicable Applications for anti-PFKL antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 700-750 of Human PFKL.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of PFKL (E724) pAb in paraffin-embedded human brain tissue.)

Related Product Information for anti-PFKL antibody

Phosphofructokinase (PFK) catalyzes the phosphorylation of fructose-6-phosphate in glycolysis. There are three isozymes: muscle-type, liver-type, and platelet-type. The liver-type PFK (PFKL) is the predominant subunit of PFK found in the liver. Research studies have shown that because of its location on chromosome 21, this enzyme is overexpressed in Down Syndrome/trisomy 21. PFKL also may have a role in the physiological response to a low fat diet.

NCBI and Uniprot Product Information

• NCBI GI #: 48762920
• NCBI GeneID: 5211
• NCBI Accession #: NP_002617.3
• NCBI GenBank Nucleotide #: NP_002617.3
• UniProt Accession #: P17858
• Molecular Weight: ~ 85kDa
• NCBI Official Full Name: ATP-dependent 6-phosphofructokinase, liver type isoform b
• NCBI Official Synonym Full Names: phosphofructokinase, liver type
• NCBI Official Symbol: PFKL
• NCBI Official Synonym Symbols: PFK-B; PFK-L; ATP-PFK
• NCBI Protein Information: ATP-dependent 6-phosphofructokinase, liver type
• UniProt Protein Name: 6-phosphofructokinase, liver type
• Protein Family: ATP-dependent 6-phosphofructokinase
• UniProt Gene Name: PFKL
• UniProt Synonym Gene Names: PFK-B
• UniProt Entry Name: K6PL_HUMAN

NCBI Description

This gene encodes the liver (L) subunit of an enzyme that catalyzes the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate, which is a key step in glucose metabolism (glycolysis). This enzyme is a tetramer that may be composed of different subunits encoded by distinct genes in different tissues. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Uniprot Description

PFKL: phosphofructokinase, liver type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts. Two alternatively spliced isoforms have been described.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pentose phosphate pathway; EC 2.7.1.11; Carbohydrate Metabolism - galactose; Kinase, other; Carbohydrate Metabolism - fructose and mannose

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: 6-phosphofructokinase complex; membrane; cytosol

Molecular Function: identical protein binding; protein binding; metal ion binding; kinase binding; ATP binding; 6-phosphofructokinase activity

Biological Process: fructose 1,6-bisphosphate metabolic process; glycolysis; carbohydrate metabolic process; carbohydrate phosphorylation; response to glucose stimulus; glucose metabolic process; pathogenesis; fructose 6-phosphate metabolic process; protein oligomerization; protein homotetramerization; negative regulation of insulin secretion