Rat Matrix Metalloproteinase 3 (MMP3) CLIA Kit | MMP3 clia kit

Rat Matrix Metalloproteinase 3 (MMP3) CLIA Kit

Cat. Num. AAA2708651

• Reactivity: Rat
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Matrix Metalloproteinase 3 (MMP3); Rat Matrix Metalloproteinase 3 (MMP3) CLIA Kit; Matrix Metalloproteinase 3; SL1; STMY; STMY1; STR1; Progelatinase; Stromelysin 1; Transin-1; MMP3 clia kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 3 (MMP3).; No significant cross-reactivity or interference between Matrix Metalloproteinase 3 (MMP3) and analogues was observed.
• Sequence Length: 475

• Applicable Applications for MMP3 clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 27.4-20,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 12.1pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 3 (MMP3) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 3 (MMP3) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 3 (MMP3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 3 (MMP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Matrix Metalloproteinase 3 (MMP3) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 116860
• NCBI GeneID: 171045
• UniProt Accession #: P03957
• NCBI Official Full Name: Stromelysin-1
• NCBI Official Synonym Full Names: matrix metallopeptidase 3
• NCBI Official Symbol: Mmp3
• NCBI Protein Information: stromelysin-1
• UniProt Protein Name: Stromelysin-1
• Protein Family: Stromelysin
• UniProt Gene Name: Mmp3
• UniProt Synonym Gene Names: SL-1; MMP-3

NCBI Description

endopeptidase that degrades extracellular matrix proteins [RGD, Feb 2006]

Uniprot Description

Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.