Western Blot (WB)
((0. 01ug/ml) staining of peripheral blood mononucleocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat LAT1 Polyclonal Antibody | anti-LAT antibody
Goat anti-LAT1 Antibody
Western Blot: Approx 37kDa band observed in Human Lymph Node and Peripheral Brood Mononucleocytes lysates and in Mouse and Rat Thymus lysates (calculated MW of 27.9kDa according to NP_055202.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.01-0.03ug/ml.
Western Blot (WB)
((0. 01ug/ml) staining of peripheral blood mononucleocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 01ug/ml) staining of peripheral blood mononucleocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
LAT: an adaptor protein involved in T-cell antigen receptor (TCR) signal transduction pathway. Plays an important role downstream of protein tyrosine kinases. Essential for TCR-mediated Ras-Erk activation and calcium flux.
Protein type: Adaptor/scaffold; Membrane protein, integral
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: immunological synapse; integral to membrane; lipid raft; plasma membrane; signalosome
Molecular Function: protein binding; protein kinase binding; Ras guanyl-nucleotide exchange factor activity; SH3/SH2 adaptor activity
Biological Process: calcium-mediated signaling; immune response; integrin-mediated signaling pathway; MAPKKK cascade; platelet activation; positive regulation of protein kinase activity; Ras protein signal transduction; regulation of T cell activation; T cell activation; T cell receptor signaling pathway
Research Articles on LAT
Similar Products
Product Notes
The LAT lat (Catalog #AAA420583) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-LAT1 Antibody reacts with Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's LAT1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 2000. Western Blot: Approx 37kDa band observed in Human Lymph Node and Peripheral Brood Mononucleocytes lysates and in Mouse and Rat Thymus lysates (calculated MW of 27.9kDa according to NP_055202.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.01-0.03ug/ml. Researchers should empirically determine the suitability of the LAT lat for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GAPDYENLQE LN. It is sometimes possible for the material contained within the vial of "LAT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
