Typical Testing Data/Standard Curve (for reference only)
Rat E-selectin CLIA Kit | Sele clia kit
Rat E-selectin CLIA Kit
Reactivity
Rat
Applications
Chemiluminescence Immunoassay (CLIA)
Synonyms
E-selectin; Rat E-selectin CLIA Kit; CD62E; CD62-E; E-Selectin; ELAM; ELAM1; ESEL; SEL-E; E-LAM; E-LAM1; E-SEL; LECAM2; Endothelial Leukocyte Adhesion Molecule 1; CD62 Antigen-Like Family Member E; Sele clia kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of E-selectin.
No significant cross-reactivity or interference between E-selectin and analogues was observed.
No significant cross-reactivity or interference between E-selectin and analogues was observed.
Sequence Length
549
Applicable Applications for Sele clia kit
Chemiluminescence Immunoassay (CLIA)
Assay Type
Double-antibody Sandwich
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
2.74-2,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 1.24pg/mL
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level E-selectin were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level E-selectin were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level E-selectin were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Test Principle
The microplate provided in this kit has been pre-coated with an antibody specific to E-selectin. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to E-selectin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the E-selectin level in the sample or standard.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
E-selectin
NCBI Official Synonym Full Names
selectin E
NCBI Official Symbol
Sele
NCBI Protein Information
E-selectin
UniProt Protein Name
E-selectin
Protein Family
UniProt Gene Name
Sele
UniProt Synonym Gene Names
Elam-1; ELAM-1; LECAM2
UniProt Entry Name
LYAM2_RAT
NCBI Description
facilitates recruitment of leukocytes into sites of inflammation; plays a role in cell adhesion [RGD, Feb 2006]
Uniprot Description
Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis ().
Research Articles on Sele
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Product Notes
The Rat Sele sele (Catalog #AAA2708493) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2708493 CLIA Kit recognizes Rat Sele. AAA Biotech's E-selectin can be used in a range of immunoassay formats including, but not limited to, Chemiluminescence Immunoassay (CLIA). Researchers should empirically determine the suitability of the Sele sele for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "E-selectin, CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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