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Western Blot (WB) (Western blot analysis Netrin-1 using LOVO whole cell lysates)

Rabbit Netrin-1 Polyclonal Antibody | anti-NTN1 antibody

Netrin-1 Antibody

Gene Names
NTN1; NTN1L
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
Netrin-1; Polyclonal Antibody; Netrin-1 Antibody; epididymis tissue protein Li 131P; NET1_HUMAN; Netrin 1; mouse; homolog of; Netrin 1-like; Netrin1; Ntn 1; NTN 1L; NTN1; NTN1L; Unc6; anti-NTN1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Netrin-1 antibody detects endogenous levels of total Netrin-1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
604
Applicable Applications for anti-NTN1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
Immunogen
A synthesized peptide derived from human Netrin-1
Subcellular Location
Extracellular Region or Secreted
Tissue Specificity
Widely expressed in normal adult tissues with highest levels in heart, small intestine, colon, liver and prostate. Reduced expression in brain tumors and neuroblastomas. Expressed in epididymis (at protein level).
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Chicken, Xenopus
Similarity
Pig (100%), Bovine (100%), Horse (90%), Sheep (100%), Chicken (90%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis Netrin-1 using LOVO whole cell lysates)

Western Blot (WB) (Western blot analysis Netrin-1 using LOVO whole cell lysates)

Western Blot (WB)

(Western blot analysis of extracts from mouse brain, using Netrin-1 Antibody. Lane 1 was treated with the antigen-specific peptide.)

Western Blot (WB) (Western blot analysis of extracts from mouse brain, using Netrin-1 Antibody. Lane 1 was treated with the antigen-specific peptide.)

Immunohistochemistry (IHC)

(MBS9608896 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Immunohistochemistry (IHC) (MBS9608896 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-NTN1 antibody
Function: Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in tumorigenesis by regulating apoptosis.
Subunit Structure: Binds to its receptors; DCC, DSCAM, UNC5A, UNC5B, UNC5C and probably UNC5D.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 67 kDa
Predicted: 68 kDa
NCBI Official Full Name
netrin-1
NCBI Official Synonym Full Names
netrin 1
NCBI Official Symbol
NTN1
NCBI Official Synonym Symbols
NTN1L
NCBI Protein Information
netrin-1
UniProt Protein Name
Netrin-1
Protein Family
UniProt Gene Name
NTN1
UniProt Synonym Gene Names
NTN1L

NCBI Description

Netrin is included in a family of laminin-related secreted proteins. The function of this gene has not yet been defined; however, netrin is thought to be involved in axon guidance and cell migration during development. Mutations and loss of expression of netrin suggest that variation in netrin may be involved in cancer development. [provided by RefSeq, Jul 2008]

Uniprot Description

Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in tumorigenesis by regulating apoptosis.

Research Articles on NTN1

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Product Notes

The NTN1 ntn1 (Catalog #AAA9608896) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Netrin-1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Netrin-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200. Researchers should empirically determine the suitability of the NTN1 ntn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Netrin-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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