SDS-Page
Rala recombinant protein
Rala, 1-203aa, Mouse, His-tag, E Coli
Gene Names
Rala; Ral; Rasl1; AW322615; 3010001O15Rik
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
Rala; 1-203aa; Mouse; His-tag; E Coli; Ras-related protein Ral-A; 3010001O15Rik; AW322615; Ral; Rasl1; Rala recombinant protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.5) containing 30% glycerol, 0.1M NaCl, 0.1mM PMSF
Concentration
0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAANKP KGQNSLALHK VIMVGSGGVG KSALTLQFMY DEFVEDYEPT KADSYRKKVV LDGEEVQIDI LDTAGQEDYA AIRDNYFRSG EGFLCVFSIT EMESFAATAD FREQILRVKE DENVPFLLVG NKSDLEDKRQ VSVEEAKNRA DQWNVNYVET SAKTRANVDK VFFDLMREIR ARKMEDSKEK NGKKKRKSLA KRIRERC
Sequence Length
206
Applicable Applications for Rala recombinant protein
SDS-PAGE
Antigen Species
Mouse
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for Rala recombinant protein
Rala, also known as Ras-related protein Ral-A, is a member of the small GTPase superfamily, Ras family of proteins. Rala mediates a distinct downstream signaling pathway from Ras that facilitates cellular transformation. Rala is also thought to be involved in numerous signaling cascades, including regulation of the cytoskeleton, vesicle trafficking, and endocytosis. Recombinant mouse Rala protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for Rala recombinant protein
References
Cascone I., et al. (2008) EMBO J. 27 (18):2375-2387.; Balasubramanian N., et al. (2010) Curr. Biol. 20 (1):75-79.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25.7 kDa (227aa) confirmed by MALDI-TOF
NCBI Official Full Name
ras-related protein Ral-A
NCBI Official Synonym Full Names
v-ral simian leukemia viral oncogene A (ras related)
NCBI Official Symbol
Rala
NCBI Official Synonym Symbols
Ral; Rasl1; AW322615; 3010001O15Rik
NCBI Protein Information
ras-related protein Ral-A
UniProt Protein Name
Ras-related protein Ral-A
Protein Family
UniProt Gene Name
Rala
UniProt Synonym Gene Names
Ral; Ral-a
Uniprot Description
Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Key regulator of LPAR1 signaling and competes with GRK2 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells (). The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling (PubMed:20005108). During mitosis, supports the stabilization and elongation of the intracellular bridge between dividing cells. Cooperates with EXOC2 to recruit other components of the exocyst to the early midbody ().
Research Articles on Rala
Similar Products
Product Notes
The Rala rala (Catalog #AAA206749) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's Rala can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the Rala rala for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSHMAANKP KGQNSLALHK VIMVGSGGVG KSALTLQFMY DEFVEDYEPT KADSYRKKVV LDGEEVQIDI LDTAGQEDYA AIRDNYFRSG EGFLCVFSIT EMESFAATAD FREQILRVKE DENVPFLLVG NKSDLEDKRQ VSVEEAKNRA DQWNVNYVET SAKTRANVDK VFFDLMREIR ARKMEDSKEK NGKKKRKSLA KRIRERC. It is sometimes possible for the material contained within the vial of "Rala, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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