Ribosome-associated inhibitor A (raiA) Recombinant Protein | raiA recombinant protein

Recombinant Escherichia coli Ribosome-associated inhibitor A (raiA)

Cat. Num. AAA1075604

• Gene Names: raiA; ECK2594; JW2578; yfiA
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Ribosome-associated inhibitor A (raiA); Recombinant Escherichia coli Ribosome-associated inhibitor A (raiA); raiA recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 2-113, full length protein
• Sequence: TMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKEPQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVKDANFVEEVEEE
• Sequence Length: 112

• Species: Escherichia coli (strain K12)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 16130518
• NCBI GeneID: 947129
• NCBI Accession #: NP_417088.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P0AD49; P11285
• Molecular Weight: 12,785 Da
• NCBI Official Full Name: cold shock protein associated with 30S ribosomal subunit
• NCBI Official Symbol: raiA
• NCBI Official Synonym Symbols: ECK2594; JW2578; yfiA
• NCBI Protein Information: cold shock protein associated with 30S ribosomal subunit
• UniProt Protein Name: Ribosome-associated inhibitor A
• Protein Family: Ribosome-associated inhibitor
• UniProt Gene Name: raiA
• UniProt Synonym Gene Names: pY

NCBI Description

RaiA is a ribosome-associated factor that is induced in stationary phase. hpf paralog has opposite effect on 70S dimers. [More information is available at EcoGene: EG11151]. YfiA interferes with translation elongation, specifically by occluding the access of aminoacyl-tRNA to the ribosomal A site . [More information is available at EcoCyc: EG11151].

Uniprot Description

During stationary phase prevents 70S dimer formation, probably in order to regulate translation efficiency during transition between the exponential and the stationary phases (PubMed:16324148). During environmental stress such as cold shock or excessive cell density at stationary phase, stabilizes the 70S ribosome against dissociation, inhibits translation elongation and increases translation accuracy (PubMed:11375931, PubMed:15219834). When normal growth conditions are restored, is quickly released from the ribosome (PubMed:11375931). Has been suggested to inhibit translation elongation by blocking the A-site (aminoacyl-tRNA site) (PubMed:11375931). Has also been suggested to inhibit translation initiation by blocking the A-site and P-site (peptidyl-tRNA site) of the ribosome (PubMed:15502846, PubMed:23420694). At 15 degrees Celsius binds 30S subunits and stimulates their association with 50S subunits into idle 70S ribosomes (PubMed:23420694). Crystallization with T.thermophilus 70S ribosomes shows it binds in the channel between the head and body of the 30S subunit, where mRNA, tRNAs, initiation factors IF1 and IF3 and elongation factor G would bind; this protein's extended tail follows the mRNA channel and probably prevents RMF binding, which would prevent ribosome dimerization (PubMed:22605777). This protein also stabilizes the 30S head relative to the rest of the ribosome, which may also prevent dimerization (PubMed:22605777). Counteracts miscoding (translation errors) particularly efficiently at magnesium concentrations close to those observed in vivo but less efficiently at higher concentrations (PubMed:15219834). Counteraction of miscoding was shown to be stronger than inhibition of translation, suggesting that the former activity could be the main function of this protein in vivo (PubMed:15219834).