Rabbit anti-Human, Mouse MAGOH Polyclonal Antibody | anti-MAGOH antibody

MAGOH Antibody

Cat. Num. AAA7045809

• Gene Names: MAGOH; MAGOH1; MAGOHA
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Antigen Affinity Purified
• Synonyms: MAGOH; Polyclonal Antibody; MAGOH Antibody; Protein mago nashi homolog; MAGOHA; anti-MAGOH antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 146

• Applicable Applications for anti-MAGOH antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Species: Human
• Immunogen: Recombinant human Protein mago nashi homolog protein (1-146AA)
• Conjugate: Non-conjugated
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-48237 / sc-48239 / sc-48240 / sc-99124
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: MAGOH antibody at 4.69 ug/mlLane 1: Mouse kidney tissueLane 2: A431 whole cell lysateLane 3: Jurkat whole cell lysateLane 4: Raji whole cell lysateLane 5: K562 whole cell lysateLane 6: Hela whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 18,13 kDaObserved band size: 17 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human ovarian cancer using MBS7045809 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7045809 at dilution of 1:100)

Immunoprecipitation (IP)

(Immunoprecipitating MAGOH in HeLa whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of MBS7045809 in HeLa whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: MBS7045809(4ug)+ HeLa whole cell lysate(500ug)Lane 3: HeLa whole cell lysate (20ug))

Related Product Information for anti-MAGOH antibody

Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly.

NCBI and Uniprot Product Information

• NCBI GI #: 4505087
• NCBI GeneID: 4116
• NCBI Accession #: NP_002361.1
• NCBI GenBank Nucleotide #: NM_002370.3
• UniProt Accession #: P61326; O35169; P50606; Q5SW69; B1ARP8; B2R5A2
• Molecular Weight: 12,844 Da
• NCBI Official Full Name: protein mago nashi homolog
• NCBI Official Synonym Full Names: mago homolog, exon junction complex core component
• NCBI Official Symbol: MAGOH
• NCBI Official Synonym Symbols: MAGOH1; MAGOHA
• NCBI Protein Information: protein mago nashi homolog
• UniProt Protein Name: Protein mago nashi homolog
• Protein Family: Protein mago nashi
• UniProt Gene Name: MAGOH
• UniProt Synonym Gene Names: MAGOHA
• UniProt Entry Name: MGN_HUMAN

NCBI Description

Drosophila that have mutations in their mago nashi (grandchildless) gene produce progeny with defects in germplasm assembly and germline development. This gene encodes the mammalian mago nashi homolog. In mammals, mRNA expression is not limited to the germ plasm, but is expressed ubiquitously in adult tissues and can be induced by serum stimulation of quiescent fibroblasts. [provided by RefSeq, Jul 2008]

Uniprot Description

MAGOH: Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Remains associated with the mRNA after its export to the cytoplasm and require translation of the mRNA for removal. The heterodimer MAGOH-RBM8A interacts with PYM that function to enhance the translation of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Belongs to the mago nashi family.

Protein type: Spliceosome; RNA splicing

Chromosomal Location of Human Ortholog: 1p32.3

Cellular Component: cytosol; nucleoplasm; nucleus

Molecular Function: protein binding

Biological Process: mRNA 3'-end processing; mRNA catabolic process, nonsense-mediated decay; mRNA export from nucleus; nuclear mRNA splicing, via spliceosome; regulation of alternative nuclear mRNA splicing, via spliceosome; RNA export from nucleus; termination of RNA polymerase II transcription