Human DKK-4 ELISA Kit | DKK-4 elisa kit

Human DKK-4 PicoKine ELISA Kit

Cat. Num. AAA1751544

• Gene Names: DKK4; DKK-4
• Reactivity: Human
• Synonyms: DKK-4; Human DKK-4 PicoKine ELISA Kit; Human DKK-4; DKK-4 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human DEFA1
• Sequence Length: 224

• Samples: Cell Culture Supernates, Serum And Plasma (Heparin, Edta)
• Detection Range: 156pg/ml-10000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DKK-4 elisa kit

Principle of the Assay: The Picokine Human DEFA1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human DEFA1 with a 96-well strip plate that is pre-coated with antibody specific for DEFA1. The detection antibody is a biotinylated antibody specific for DEFA1. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human DEFA1 with immunogen: Expression system for standard: E.coli,D39-C94. The kit is analytically validated with ready to use reagents. To measure Human DEFA1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human DEFA1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human DEFA1 in the sample!!Background/Introduction: Defensin is a human protein that is encoded by the DEFA1 gene. Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation.

NCBI and Uniprot Product Information

• NCBI GI #: 7657025
• NCBI GeneID: 27121
• NCBI Accession #: NP_055235.1
• NCBI GenBank Nucleotide #: NM_014420.3
• UniProt Accession #: Q9UBT3
• NCBI Official Full Name: dickkopf-related protein 4
• NCBI Official Synonym Full Names: dickkopf WNT signaling pathway inhibitor 4
• NCBI Official Symbol: DKK4
• NCBI Official Synonym Symbols: DKK-4
• NCBI Protein Information: dickkopf-related protein 4

NCBI Description

This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. Activity of this protein is modulated by binding to the Wnt co-receptor and the co-factor kremen 2. [provided by RefSeq, Jul 2008]