Rabbit Recombination activating gene 2 ELISA Kit | RAG-2 elisa kit

Rabbit Recombination activating gene 2 ELISA Kit

Cat. Num. AAA720824

• Gene Names: PAG6; PAG2; PAG10
• Reactivity: Rabbit
• Synonyms: Recombination activating gene 2; Rabbit Recombination activating gene 2 ELISA Kit; RAG-2 elisa kit

• Reactivity: Rabbit

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 10-250ng/mL
• Sensitivity: 1.0ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RAG-2 elisa kit

For Samples: Cell culture fluid, body fluid, tissue homogenate, serum and blood plasma

Intended Uses: This RAG2 ELISA kit is intended for laboratory research use only. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of RAG2 in the sample, this RAG2 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus RAG2 concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of RAG2 utilizes a multiclonal anti-RAG2 antibody and an RAG2-HRP conjugate. The assay sample and buffer are incubated together with RAG2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RAG2 concentration since RAG2 from samples and RAG2-HRP conjugate compete for the anti-RAG2 antibody binding site. Since the number of sites is limited, as more sites are occupied by RAG2 from the sample, fewer sites are left to bind RAG2-HRP conjugate. Standards of known RAG2 concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of RAG2. The RAG2 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 55741443
• NCBI GeneID: 396749
• NCBI Accession #: NP_001001536.2
• NCBI GenBank Nucleotide #: NM_001001536.2
• Molecular Weight: 47,132 Da
• NCBI Official Full Name: pregnancy-associated glycoprotein 2
• NCBI Official Symbol: PAG6
• NCBI Official Synonym Symbols: PAG2; PAG10
• NCBI Protein Information: pregnancy-associated glycoprotein 2; PAG 2; aspartic proteinase; pregnancy-associated glycoprotein 10
• UniProt Protein Name: Pregnancy-associated glycoprotein 2
• UniProt Gene Name: PAG2
• UniProt Synonym Gene Names: PAG 2
• UniProt Entry Name: PAG2_PIG