Western Blot (WB)
(Anti-Aquaporin 5 antibody, MBS175122, Western blottingWorking concentration: 0.5ug/mlLane 1: Rat Lung Tissue LysateLane 2: Rat Testis Tissue Lysate )
Rabbit Aquaporin 5 Polyclonal Antibody | anti-AQP5 antibody
Anti-Aquaporin 5 antibody
Reactivity
Reacts with : RatPredicted to work with : Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Synonyms
Aquaporin 5; Polyclonal Antibody; Anti-Aquaporin 5 antibody; Aquaporin-5(AQP-5); aquaporin 5; AQP 5 antibody; AQP-5 antibody; AQP5 antibody; AQP5_HUMAN antibody; Aquaporin 5 antibody; Aquaporin-5 antibody; anti-AQP5 antibody
Host
Rabbit
Reactivity
Reacts with : Rat
Predicted to work with : Mouse
Predicted to work with : Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
265
Applicable Applications for anti-AQP5 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes
WB: Concentration: 0.1-0.5ug/mL ; Tested Species: Rat; Predicted Species: Mouse
IHC: Concentration: 0.5-0.1ug/mL; Tested Species: Rat; Predicted Species: Mouse; Antigen Retrieval: By Heat
Tested Species: In-house tested species with positive results
Predicted Species: Species predicted to be fit for the product based on sequence similarities
By Heat: Boiling the paraffin sections in 10mM citratebuffer , pH6.0, for 20 mins is required for the staining of formalin/paraffin sections
Other applicaitons have not been tested
Optimal dilutions should be determined by the end users.
IHC: Concentration: 0.5-0.1ug/mL; Tested Species: Rat; Predicted Species: Mouse; Antigen Retrieval: By Heat
Tested Species: In-house tested species with positive results
Predicted Species: Species predicted to be fit for the product based on sequence similarities
By Heat: Boiling the paraffin sections in 10mM citratebuffer , pH6.0, for 20 mins is required for the staining of formalin/paraffin sections
Other applicaitons have not been tested
Optimal dilutions should be determined by the end users.
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of mouse Aquaporin 5 (246-265aa EEDWEDHREERKKTIELTAH), identical to the related rat sequence.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti-Aquaporin 5 antibody, MBS175122, Western blottingWorking concentration: 0.5ug/mlLane 1: Rat Lung Tissue LysateLane 2: Rat Testis Tissue Lysate )
Western Blot (WB)
(Anti-Aquaporin 5 antibody, MBS175122, Western blottingWorking concentration: 0.5ug/mlLane 1: Rat Lung Tissue LysateLane 2: Rat Testis Tissue Lysate )
Immunohistochemistry (IHC)
(Anti-Aquaporin 5 antibody, MBS175122, IHC(P)IHC(P): Rat Lung Tissue LysateWorking concentration: 1ug/ml )
Immunohistochemistry (IHC)
(Anti-Aquaporin 5 antibody, MBS175122, IHC(P)IHC(P): Rat Lung Tissue LysateWorking concentration: 1ug/ml )
Related Product Information for anti-AQP5 antibody
Description: Rabbit IgG polyclonal antibody for Aquaporin-5(AQP5) detection. Tested with WB, IHC-P in Mouse, Rat.
Background: Aquaporin 5, also known as AQP5, is a water channel protein. The aquaporins(AQPs) are a family of more than 10 homologous water transporting proteins expressed in many mammalian epithelia and endothelia. At least five AQPs are expressed in the eye: AQP0(MIP) in lens fiber, AQP1 in cornea endothelium, ciliary and lens epithelia and trabecular meshwork, AQP3 in conjunctiva, AQP4 in ciliary epithelium and retinal Müller cells, and AQP5 in corneal and lacrimal gland epithelia. Among the seven human aquaporins cloned to date(AQPs 0-6), genes encoding the four most closely related aquaporins(AQP0, AQP2, AQP5, and AQP6) have been mapped to chromosome band 12q13, suggesting an aquaporin family gene cluster at this locus. Aquaporin 5 plays a role in the generation of saliva, tears and pulmonary secretions.
Background: Aquaporin 5, also known as AQP5, is a water channel protein. The aquaporins(AQPs) are a family of more than 10 homologous water transporting proteins expressed in many mammalian epithelia and endothelia. At least five AQPs are expressed in the eye: AQP0(MIP) in lens fiber, AQP1 in cornea endothelium, ciliary and lens epithelia and trabecular meshwork, AQP3 in conjunctiva, AQP4 in ciliary epithelium and retinal Müller cells, and AQP5 in corneal and lacrimal gland epithelia. Among the seven human aquaporins cloned to date(AQPs 0-6), genes encoding the four most closely related aquaporins(AQP0, AQP2, AQP5, and AQP6) have been mapped to chromosome band 12q13, suggesting an aquaporin family gene cluster at this locus. Aquaporin 5 plays a role in the generation of saliva, tears and pulmonary secretions.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Aquaporin-5
NCBI Official Synonym Full Names
aquaporin 5
NCBI Official Symbol
Aqp5
NCBI Protein Information
aquaporin-5; AQP-5
UniProt Protein Name
Aquaporin-5
Protein Family
UniProt Gene Name
Aqp5
UniProt Synonym Gene Names
AQP-5
UniProt Entry Name
AQP5_MOUSE
Uniprot Description
AQP5: Forms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions. Belongs to the MIP/aquaporin (TC 1.A.8) family.
Protein type: Transporter; Transporter, aquaporin family; Membrane protein, integral; Membrane protein, multi-pass; Channel, misc.
Cellular Component: microvillus; membrane; endoplasmic reticulum; integral to plasma membrane; cell; apical plasma membrane; integral to membrane; plasma membrane; basal plasma membrane
Molecular Function: protein binding; transporter activity; water channel activity; glycerol channel activity
Biological Process: glycerol transport; camera-type eye morphogenesis; cellular water homeostasis; transport; carbon dioxide transport; cellular response to stress; water transport; transmembrane transport; saliva secretion
Research Articles on AQP5
Similar Products
Product Notes
The AQP5 aqp5 (Catalog #AAA175122) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Aquaporin 5 antibody reacts with Reacts with : Rat Predicted to work with : Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Aquaporin 5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. WB: Concentration: 0.1-0.5ug/mL ; Tested Species: Rat; Predicted Species: Mouse IHC: Concentration: 0.5-0.1ug/mL; Tested Species: Rat; Predicted Species: Mouse; Antigen Retrieval: By Heat Tested Species: In-house tested species with positive results Predicted Species: Species predicted to be fit for the product based on sequence similarities By Heat: Boiling the paraffin sections in 10mM citratebuffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections Other applicaitons have not been tested Optimal dilutions should be determined by the end users. Researchers should empirically determine the suitability of the AQP5 aqp5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aquaporin 5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
