Human Recombination activating gene 1 ELISA Kit | PAG 1 elisa kit

Human Recombination activating gene 1 ELISA Kit

Cat. Num. AAA730374

• Gene Names: PAG1; PAG1B
• Reactivity: Human
• Synonyms: Recombination activating gene 1; Human Recombination activating gene 1 ELISA Kit; PAG 1 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 50-1000pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This PAG-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PAG-1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PAG 1 elisa kit

Principle of the Assay: PAG-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PAG-1. Standards or samples are then added to the microtiter plate wells and PAG-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PAG-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for PAG-1 are added to each well to "sandwich" the PAG-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PAG-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAG-1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PAG 1 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 27806043
• NCBI GeneID: 281964
• NCBI Accession #: NP_776836.1
• NCBI GenBank Nucleotide #: NM_174411.2
• Molecular Weight: 42,847 Da
• NCBI Official Full Name: pregnancy-associated glycoprotein 1
• NCBI Official Symbol: PAG1
• NCBI Official Synonym Symbols: PAG1B
• NCBI Protein Information: pregnancy-associated glycoprotein 1; PAG 1; PSP-B; pregnancy-specific protein B
• UniProt Protein Name: Pregnancy-associated glycoprotein 1
• UniProt Gene Name: PAG 1
• UniProt Synonym Gene Names: PSP-B
• UniProt Entry Name: PAG1_BOVIN

Uniprot Description

Appears to be proteolytically inactive.