Typical Testing Data/Standard Curve (for reference only)
Human Retinoic Acid Early Transcript 1L ELISA Kit | RAET1L elisa kit
Human Retinoic Acid Early Transcript 1L ELISA Kit
Gene Names
RAET1L; ULBP6
Reactivity
Human
Synonyms
Retinoic Acid Early Transcript 1L; Human Retinoic Acid Early Transcript 1L ELISA Kit; RAET1L elisa kit
Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
1.0-25ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for RAET1L elisa kit
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
INTENDED USE This BG RAET1L ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human RAET1L. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY RAET1L ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RAET1L antibody and an RAET1L-HRP conjugate. The assay sample and buffer are incubated together with RAET1L-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RAET1L concentration since RAET1L from samples and RAET1L-HRP conjugate compete for the anti-RAET1L antibody binding site. Since the number of sites is limited, as more sites are occupied by RAET1L from the sample, fewer sites are left to bind RAET1L-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RAET1L concentration in each sample is interpolated from this standard curve.
INTENDED USE This BG RAET1L ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human RAET1L. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY RAET1L ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RAET1L antibody and an RAET1L-HRP conjugate. The assay sample and buffer are incubated together with RAET1L-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RAET1L concentration since RAET1L from samples and RAET1L-HRP conjugate compete for the anti-RAET1L antibody binding site. Since the number of sites is limited, as more sites are occupied by RAET1L from the sample, fewer sites are left to bind RAET1L-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RAET1L concentration in each sample is interpolated from this standard curve.
Product Categories/Family for RAET1L elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,509 Da
NCBI Official Full Name
retinoic acid early transcript 1L protein
NCBI Official Synonym Full Names
retinoic acid early transcript 1L
NCBI Official Symbol
RAET1L
NCBI Official Synonym Symbols
ULBP6
NCBI Protein Information
retinoic acid early transcript 1L protein; UL16 binding protein 6
UniProt Protein Name
Retinoic acid early transcript 1L protein
Protein Family
UniProt Gene Name
RAET1L
UniProt Entry Name
RET1L_HUMAN
NCBI Description
RAET1L belongs to the RAET1 family of major histocompatibility complex (MHC) class I-related genes, which are located within a 180-kb cluster on chromosome 6q24.2-q25.3. The REAT1 genes encode glycoproteins that contain extracellular alpha-1 and alpha-2 domains, but they lack the membrane proximal Ig-like alpha-3 domain. Most RAET1 glycoproteins are anchored to the membrane via glycosylphosphatidylinositol (GPI) linkage (Radosavljevic et al., 2002 [PubMed 11827464]).[supplied by OMIM, Mar 2008]
Uniprot Description
RAET1L: Belongs to the MHC class I family
Protein type: Membrane protein, GPI anchor
Chromosomal Location of Human Ortholog: 6q25.1
Cellular Component: plasma membrane
Molecular Function: peptide antigen binding; natural killer cell lectin-like receptor binding
Biological Process: antigen processing and presentation of peptide antigen via MHC class I; natural killer cell mediated cytotoxicity
Research Articles on RAET1L
Similar Products
Product Notes
The Human RAET1L raet1l (Catalog #AAA732277) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA732277 ELISA Kit recognizes Human RAET1L. It is sometimes possible for the material contained within the vial of "Retinoic Acid Early Transcript 1L, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
