Sheep Retinoic Acid Early Transcript 1L ELISA Kit | RAET1L elisa kit

Sheep Retinoic Acid Early Transcript 1L ELISA Kit

Cat. Num. AAA755206

• Gene Names: RAET1L; ULBP6
• Reactivity: Sheep
• Synonyms: Retinoic Acid Early Transcript 1L; Sheep Retinoic Acid Early Transcript 1L ELISA Kit; RAET1L elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This RAET1L ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep RAET1L. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RAET1L elisa kit

Principle of the assay: RAET1L ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RAET1L antibody and an RAET1L-HRP conjugate. The assay sample and buffer are incubated together with RAET1L-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RAET1L concentration since RAET1L from samples and RAET1L-HRP conjugate compete for the anti-RAET1L antibody binding site. Since the number of sites is limited, as more sites are occupied by RAET1L from the sample, fewer sites are left to bind RAET1L-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RAET1L concentration in each sample is interpolated from this standard curve.

Product Categories/Family for RAET1L elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 126723295
• NCBI GeneID: 154064
• NCBI Accession #: NP_570970.2
• NCBI GenBank Nucleotide #: NM_130900.2
• Molecular Weight: 27,509 Da
• NCBI Official Full Name: retinoic acid early transcript 1L protein
• NCBI Official Synonym Full Names: retinoic acid early transcript 1L
• NCBI Official Symbol: RAET1L
• NCBI Official Synonym Symbols: ULBP6
• NCBI Protein Information: retinoic acid early transcript 1L protein; UL16 binding protein 6
• UniProt Protein Name: Retinoic acid early transcript 1L protein
• Protein Family: Retinoic acid early transcript 1L protein
• UniProt Gene Name: RAET1L
• UniProt Entry Name: RET1L_HUMAN

NCBI Description

RAET1L belongs to the RAET1 family of major histocompatibility complex (MHC) class I-related genes, which are located within a 180-kb cluster on chromosome 6q24.2-q25.3. The REAT1 genes encode glycoproteins that contain extracellular alpha-1 and alpha-2 domains, but they lack the membrane proximal Ig-like alpha-3 domain. Most RAET1 glycoproteins are anchored to the membrane via glycosylphosphatidylinositol (GPI) linkage (Radosavljevic et al., 2002 [PubMed 11827464]).[supplied by OMIM, Mar 2008]

Uniprot Description

RAET1L: Belongs to the MHC class I family

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 6q25.1

Cellular Component: plasma membrane

Molecular Function: peptide antigen binding; natural killer cell lectin-like receptor binding

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; natural killer cell mediated cytotoxicity