Typical Testing Data/Standard Curve (for reference only)
Rat Claudin-11 (CLDN11) ELISA Kit | CLDN11 elisa kit
Rat Claudin-11 (CLDN11) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CLDN11 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CLDN11 antibody and an CLDN11-HRP conjugate. The assay sample and buffer are incubated together with CLDN11-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLDN11 concentration since CLDN11 from samples and CLDN11-HRP conjugate compete for the anti-CLDN11 antibody binding site. Since the number of sites is limited, as more sites are occupied by CLDN11 from the sample, fewer sites are left to bind CLDN11-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLDN11 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The protein encoded by this gene is a major component of central nervous system (CNS) myelin and plays an important role in regulating proliferation and migration of oligodendrocytes. Mouse studies showed that the gene deficiency results in deafness and loss of the Sertoli cell epithelial phenotype in the testis. This protein is a tight junction protein at the human blood-testis barrier (BTB), and the BTB disruption is related to a dysfunction of this gene. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Aug 2010]
Uniprot Description
Claudin-11: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Belongs to the claudin family.
Protein type: Membrane protein, integral; Cell adhesion; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 3q26.2-q26.3
Cellular Component: tight junction; plasma membrane; integral to membrane
Molecular Function: identical protein binding; structural molecule activity
Biological Process: spermatogenesis; axon ensheathment; calcium-independent cell-cell adhesion
Research Articles on CLDN11
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Product Notes
The Rat CLDN11 cldn11 (Catalog #AAA7230633) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7230633 ELISA Kit recognizes Rat CLDN11. It is sometimes possible for the material contained within the vial of "Claudin-11 (CLDN11), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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