RAET1E recombinant protein

RAET1E, 31-225aa, Human, hIgG-His-tag, Baculovirus

Cat. Num. AAA206706

• Gene Names: RAET1E; RL-4; LETAL; ULBP4; N2DL-4; NKG2DL4; RAET1E2; bA350J20.7
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: RAET1E; 31-225aa; Human; hIgG-His-tag; Baculovirus; NKG2D ligand 4 isoform 1; bA350J20.7; LETAL; N2DL-4; NKG2DL4; RAET1E2; RL-4; ULBP4; RAET1E recombinant protein

• Host: Baculovirus
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: ADPHSLCFNF TIKSLSRPGQ PWCEAQVFLN KNLFLQYNSD NNMVKPLGLL GKKVYATSTW GELTQTLGEV GRDLRMLLCD IKPQIKTSDP STLQVEMFCQ REAERCTGAS WQFATNGEKS LLFDAMNMTW TVINHEASKI KETWKKDRGL EKYFRKLSKG DCDHWLREFL GHWEAMPEPT VSPVNASDIH WSSSSLPDVE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG KHHHHHH
• Sequence Length: 263

• Applicable Applications for RAET1E recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: hIgG-His-tag
• Endotoxin Level: < 1.0 EU per 1ug of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for RAET1E recombinant protein

RAET1E, also known as NKG2D ligand 4 isoform 1, is a member of the RAET1/ULBP family of cell surface protein that function as ligands for NKG2D. This protein is abnormally expressed on most colon cancer and some other tumor cell lines and virus infected peripheral blood cells. It binds and co-stimulates NKG2D expressing effector cells including NK cells, NKT cells, gamma delta T cells, and CD8+ alpha beta T cells, activating cytolytic activity and/or cytokine production. Also, it functions as a stress-induced ligand for NKG2D receptor. Recombinant human RAET1E, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for RAET1E recombinant protein

Immunology

References

Chalupny NJ., et al, (2003) Biochem. Biophys. Res. Commun. 305:129-135.; Kong Y., et al, (2009) Blood 114:310-317.

NCBI and Uniprot Product Information

• NCBI GI #: 21040249
• NCBI GeneID: 135250
• NCBI Accession #: NP_631904
• NCBI GenBank Nucleotide #: NM_139165.2
• UniProt Accession #: Q8TD07; Q5VYB7; Q5VYB8; Q8TEZ2; Q96L41; A6YF59
• Molecular Weight: 49.6kDa (437aa) 57-70kDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: retinoic acid early transcript 1E isoform 1
• NCBI Official Synonym Full Names: retinoic acid early transcript 1E
• NCBI Official Symbol: RAET1E
• NCBI Official Synonym Symbols: RL-4; LETAL; ULBP4; N2DL-4; NKG2DL4; RAET1E2; bA350J20.7
• NCBI Protein Information: retinoic acid early transcript 1E; NKG2D ligand 4
• UniProt Protein Name: Retinoic acid early transcript 1E
• Protein Family: Retinoic acid early-inducible protein
• UniProt Gene Name: RAET1E
• UniProt Synonym Gene Names: N2DL-4; NKG2DL4

NCBI Description

This gene belong to the RAET1 family, which consists of major histocompatibility complex (MHC) class I-related genes located in a cluster on chromosome 6q24.2-q25.3. This and RAET1G protein differ from other RAET1 proteins in that they have type I membrane-spanning sequences at their C termini rather than glycosylphosphatidylinositol anchor sequences. This protein functions as a ligand for NKG2D receptor, which is expressed on the surface of several types of immune cells, and is involved in innate and adaptive immune responses. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]

Uniprot Description

Binds and activates the KLRK1/NKG2D receptor, mediating natural killer cell cytotoxicity.