RAD51D recombinant protein

Recombinant Human RAD51D (1-328 a.a.)

Cat. Num. AAA146193

• Gene Names: RAD51D; TRAD; R51H3; BROVCA4; RAD51L3
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: RAD51D; Recombinant Human RAD51D (1-328 a.a.); RAD51D (1-328) Human; RAD51D (1-328 a.a.) Human Recombinant; BROVCA4; R51H3; RAD51L3; TRAD; DNA repair protein RAD51 homolog 4; RAD51 homolog D; RAD51-like protein 3; RAD51D (1-328); RAD51D recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: RAD51D (1-328) solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 10% glycerol and 1mM DTT.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMGVLRVG LCPGLTEEMI QLLRSHRIKT VVDLVSADLE EVAQKCGLSY KALVALRRVL LAQFSAFPVN GADLYEELKT STAILSTGIG SLDKLLDAGL YTGEVTEIVG GPGSGKTQVC LCMAANVAHG LQQNVLYVDS NGGLTASRLL QLLQAKTQDE EEQAEALRRI QVVHAFDIFQ MLDVLQELRG TVAQQVTGSS GTVKVVVVDS VTAVVSPLLG GQQREGLALM MQLARELKTL ARDLGMAVVV TNHITRDRDS GRLKPALGRS WSFVPSTRIL LDTIEGAGAS GGRRMACLAK SSRQPTGFQE MVDIGTWGTS EQSATLQGDQ T
• Sequence Length: 348

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for RAD51D recombinant protein

Description: RAD51D (1-328) Human Recombinant produced in E Coli is. a single polypeptide chain containing 351 amino acids and having a molecular mass of 37.4kDa. RAD51D (1-328) is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: RAD51D is a member of the RAD51 protein family whose members are extremely similar to bacterial RecA and Saccharomyces cerevisiae Rad51. RAD51 protein family members take part in the homologous recombination and repair of DNA. RAD51D forms a complex with several other members of the RAD51 family which catalyzes homologous pairing between single- and double-stranded DNA, and participates in the early stage of recombinational repair of DNA.

Product Categories/Family for RAD51D recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 217272816
• NCBI GeneID: 5892
• NCBI Accession #: NP_001136043.1
• NCBI GenBank Nucleotide #: NM_001142571.1
• UniProt Accession #: O75771; O43537; O60355; O75196; O75847; O75848; O76073; O76085; O94908; Q9UFU5; B4DJU7; E1P637
• Molecular Weight: 37,014 Da
• NCBI Official Full Name: DNA repair protein RAD51 homolog 4 isoform 6
• NCBI Official Synonym Full Names: RAD51 paralog D
• NCBI Official Symbol: RAD51D
• NCBI Official Synonym Symbols: TRAD; R51H3; BROVCA4; RAD51L3
• NCBI Protein Information: DNA repair protein RAD51 homolog 4; RAD51 homolog D; RAD51-like protein 3; recombination repair protein
• UniProt Protein Name: DNA repair protein RAD51 homolog 4
• Protein Family: DNA repair protein
• UniProt Gene Name: RAD51D
• UniProt Synonym Gene Names: RAD51L3
• UniProt Entry Name: RA51D_HUMAN

NCBI Description

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, which are known to be involved in the homologous recombination and repair of DNA. This protein forms a complex with several other members of the RAD51 family, including RAD51L1, RAD51L2, and XRCC2. The protein complex formed with this protein has been shown to catalyze homologous pairing between single- and double-stranded DNA, and is thought to play a role in the early stage of recombinational repair of DNA. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream ring finger and FYVE-like domain containing 1 (RFFL) gene. [provided by RefSeq, Jan 2011]

Uniprot Description

RAD51L3: Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Defects in RAD51D are a cause of susceptibility to familial breast-ovarian cancer type 4 (BROVCA4). A condition associated with familial predisposition to cancer of the breast and ovaries. Characteristic features in affected families are an early age of onset of breast cancer (often before age 50), increased chance of bilateral cancers (cancer that develop in both breasts, or both ovaries, independently), frequent occurrence of breast cancer among men, increased incidence of tumors of other specific organs, such as the prostate. Belongs to the RecA family. RAD51 subfamily. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 17q11

Cellular Component: centrosome; chromosome, telomeric region; cytoplasm; replication fork; nucleus

Molecular Function: DNA-dependent ATPase activity; protein binding; gamma-tubulin binding; four-way junction DNA binding; DNA binding; single-stranded DNA binding; ATP binding

Biological Process: meiotic recombination; strand invasion; DNA repair; telomere maintenance; double-strand break repair via homologous recombination

Disease: Breast-ovarian Cancer, Familial, Susceptibility To, 4