RACK1 / GNB2L1 recombinant protein

Recombinant Human RACK1 / GNB2L1 Protein (His & MBP tag)

Cat. Num. AAA2545494

• Gene Names: RACK1; H12.3; HLC-7; PIG21; GNB2L1; Gnb2-rs1
• Purity: > 83 % as determined by SDS-PAGE
• Synonyms: RACK1 / GNB2L1; Recombinant Human RACK1 / GNB2L1 Protein (His & MBP tag); HLC7; Gnb2-rs1; H12.3; HLC-7; PIG21; RACK1; RACK1 / GNB2L1 recombinant protein

• Host: E Coli
• Purity/Purification: > 83 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.5
• Sequence Length: 317

• Application Notes: The recombinant human GNB2L1/MBP fusion protein consists of 714 amino acids and has a calculated molecular mass of 78.7 KDa. It migrates as an approximately 70 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for RACK1 / GNB2L1 recombinant protein

Background: RACK1 (guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1), also known as GNB2L1, contains 7 WD repeats and belongs to the WD repeat G protein beta family. In the liver, RACK1 is expressed at higher levels in activated hepatic stellate cells than in hepatocytes or Kupffer cells. It is up-regulated in hepatocellular carcinomas and in the adjacent non-tumor liver tissue. RACK1 is involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. It interacts with a wide variety of proteins and plays a role in many cellular processes. GNB2L1 binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. RACK1 may recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. It inhibits the activity of SRC kinases including SRC, LCK and YES1. RACK1 also inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. It enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer.

Description: A DNA sequence encoding the human GNB2L1 (P63244) (Met 1-Arg 317) was fused with an N-terminal polyhistidine-tagged MBP tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 5174447
• NCBI GeneID: 10399
• NCBI Accession #: NP_006089.1
• NCBI GenBank Nucleotide #: NM_006098.4
• UniProt Accession #: P63244; P25388; P99049; Q53HU2; Q5J8M6; Q5VLR4; Q6FH47; B3KTJ0; D3DWS0
• Molecular Weight: 35,077 Da
• NCBI Official Full Name: receptor of activated protein C kinase 1
• NCBI Official Synonym Full Names: receptor for activated C kinase 1
• NCBI Official Symbol: RACK1
• NCBI Official Synonym Symbols: H12.3; HLC-7; PIG21; GNB2L1; Gnb2-rs1
• NCBI Protein Information: receptor of activated protein C kinase 1
• UniProt Protein Name: Receptor of activated protein C kinase 1
• UniProt Gene Name: RACK1
• UniProt Synonym Gene Names: HLC-7

Uniprot Description

RACK1: a G-beta like protein containing 7 WD domains. A presumptive scaffold protein, linking protein kinase C to its substrates. Binds inositol 1,4,5-trisphosphate receptors and regulates Ca2+ release by enhancing inositol 1,4,5-trisphosphate receptor binding affinity for inositol 1,4,5-trisphosphate. An insulin-like growth factor 1 receptor-interacting protein that can regulate IGF-1-mediated Akt activation and protection from cell death.

Protein type: Adaptor/scaffold; G protein; G protein, heterotrimeric; G protein, heterotrimeric beta; Motility/polarity/chemotaxis; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: cell soma; cell-cell adherens junction; cytoplasm; cytosol; dendrite; midbody; mitochondrion; nucleus; perinuclear region of cytoplasm; phagocytic cup; small ribosomal subunit

Molecular Function: caspase activator activity; enzyme binding; ion channel inhibitor activity; protein binding; protein complex scaffold; protein homodimerization activity; protein kinase C binding; protein phosphatase binding; protein tyrosine kinase inhibitor activity; receptor tyrosine kinase binding; SH2 domain binding

Biological Process: caspase activation; negative regulation of cell growth; negative regulation of peptidyl-serine phosphorylation; negative regulation of phagocytosis; negative regulation of protein kinase B signaling cascade; negative regulation of translation; negative regulation of Wnt receptor signaling pathway; positive regulation of apoptosis; positive regulation of cAMP catabolic process; positive regulation of cell migration; positive regulation of cyclic-nucleotide phosphodiesterase activity; positive regulation of Golgi to plasma membrane protein transport; positive regulation of GTPase activity; positive regulation of mitochondrial depolarization; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein amino acid phosphorylation; positive regulation of protein homooligomerization; regulation of cell cycle; regulation of cell division; regulation of protein localization