CD19 active protein

Human CellExp CD19, Fc Tag, Human recombinant

Cat. Num. AAA849952

• Gene Names: CD19; B4; CVID3
• Purity: >90% by SDS-PAGE
• Synonyms: CD19; Human CellExp CD19; Fc Tag; Human recombinant; B4; CVID3; MGC12802; CD19 active protein

• Host: HEK293 cells
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Lyophilized from 0.22 um filtered solution in 50mM Tris, 100mM Glycine, pH7.5. Generally Mannitol or Trehalose is added as a protectant before lyophilization

• Gene Source: Human
• Endotoxin: <1 EU/ug
• Biological Activity: Immobilized FMC63 mAb at 2 ug/mL (100 uL/well) can bind Human CD19, Fc Tag with a linear range of 0.15-1.25 ug/mL; Measured by its binding ability in a functional ELISA.
• Reconstitution Instruction: Reconstitute in sterile deionized water to a concentration of 50 ug/ml.
• Preparation and Storage: Store at -20 degree C; Centrifuge the vial prior to opening.; Shelf life: ~12 months

SDS-Page

Testing Data

Related Product Information for CD19 active protein

B-lymphocyte antigen CD19 is also known as CD19 (Cluster of Differentiation 19), is a single-pass type I membrane protein which contains two Ig-like C2-type (immunoglobulin-like) domains. CD19 is expressed on follicular dendritic cells and B cells. In fact, it is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. It primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, which leads to binding by Src-family kinases and recruitment of PI-3 kinase. As on T cells, several surface molecules form the antigen receptor and form a complex on B lymphocytes. The (almost) B cell-specific CD19 phosphoglycoprotein is one of these molecules. The others are CD21 and CD81. These surface immunoglobulin (sIg) -associated molecules facilitate signal transduction. On living B cells, anti-immunoglobulin antibody mimicking exogenous antigen causes CD19 to bind to sIg and internalize with it. The reverse process has not been demonstrated, suggesting that formation of this receptor complex is antigen-induced. This molecular association has been confirmed by chemical studies. Mutations in CD19 are associated with severe immunodeficiency syndromes characterized by diminished antibody production. CD19 has been shown to interact with: CD81, CD82, Complement receptor 2, and VAV2.

Product Categories/Family for CD19 active protein

Proteins; Enzymes Cytokines; Growth Factors & Hormones; CDs

NCBI and Uniprot Product Information

• NCBI GI #: 13623471
• NCBI GeneID: 930
• Molecular Weight: 31.6 kDa
• NCBI Official Full Name: CD19 molecule
• NCBI Official Synonym Full Names: CD19 molecule
• NCBI Official Symbol: CD19
• NCBI Official Synonym Symbols: B4; CVID3
• NCBI Protein Information: B-lymphocyte antigen CD19
• Protein Family: B-lymphocyte antigen

NCBI Description

Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008]