Rabbit GNB2L1 Polyclonal Antibody | anti-GNB2L1 antibody

GNB2L1 Antibody

Cat. Num. AAA9404948

• Gene Names: GNB2L1; H12.3; HLC-7; PIG21; RACK1; Gnb2-rs1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: GNB2L1; Polyclonal Antibody; GNB2L1 Antibody; H12.3; HLC-7; PIG21; RACK1; Gnb2-rs1; anti-GNB2L1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total GNB2L1 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 317

• Applicable Applications for anti-GNB2L1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:200; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human GNB2L1.
• Target Name: GNB2L1
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using GNB2L1 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of MCF-7 cell using GNB2L1 antibody.)

Related Product Information for anti-GNB2L1 antibody

Involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. Interacts with a wide variety of proteins and plays a role in many cellular processes. Component of the 40S ribosomal subunit involved in translational repression. Binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. May recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. Inhibits the activity of SRC kinases including SRC, LCK and YES1. Inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. Enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer. Facilitates ligand-independent nuclear translocation of AR following PKC activation, represses AR transactivation activity and is required for phosphorylation of AR by SRC. Modulates IGF1R-dependent integrin signaling and promotes cell spreading and contact with the extracellular matrix. Involved in PKC-dependent translocation of ADAM12 to the cell membrane. Promotes the ubiquitination and proteasome-mediated degradation of proteins such as CLEC1B and HIF1A. Required for VANGL2 membrane localization, inhibits Wnt signaling, and regulates cellular polarization and oriented cell division during gastrulation. Required for PTK2/FAK1 phosphorylation and dephosphorylation. Regulates internalization of the muscarinic receptor CHRM2.

Product Categories/Family for anti-GNB2L1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 5174447
• NCBI GeneID: 10399
• NCBI Accession #: NP_006089.1
• NCBI GenBank Nucleotide #: NM_006098.4
• UniProt Accession #: P63244; P25388; P99049; Q53HU2; Q5J8M6; Q5VLR4; Q6FH47; B3KTJ0; D3DWS0
• Molecular Weight: 35,077 Da
• NCBI Official Full Name: guanine nucleotide-binding protein subunit beta-2-like 1
• NCBI Official Synonym Full Names: guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1
• NCBI Official Symbol: GNB2L1
• NCBI Official Synonym Symbols: H12.3; HLC-7; PIG21; RACK1; Gnb2-rs1
• NCBI Protein Information: guanine nucleotide-binding protein subunit beta-2-like 1
• UniProt Protein Name: Guanine nucleotide-binding protein subunit beta-2-like 1
• UniProt Gene Name: GNB2L1
• UniProt Synonym Gene Names: HLC-7; RACK1
• UniProt Entry Name: GBLP_HUMAN

Uniprot Description

RACK1: a G-beta like protein containing 7 WD domains. A presumptive scaffold protein, linking protein kinase C to its substrates. Binds inositol 1,4,5-trisphosphate receptors and regulates Ca2+ release by enhancing inositol 1,4,5-trisphosphate receptor binding affinity for inositol 1,4,5-trisphosphate. An insulin-like growth factor 1 receptor-interacting protein that can regulate IGF-1-mediated Akt activation and protection from cell death.

Protein type: G protein; Adaptor/scaffold; G protein, heterotrimeric; Motility/polarity/chemotaxis; Nuclear receptor co-regulator; G protein, heterotrimeric beta

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: small ribosomal subunit; cytoskeleton; cell soma; mitochondrion; perinuclear region of cytoplasm; cytoplasm; dendrite; perikaryon; midbody; cytosol; nucleus; phagocytic cup

Molecular Function: protein tyrosine kinase inhibitor activity; protein binding; protein homodimerization activity; enzyme binding; protein kinase C binding; SH2 domain binding; protein complex scaffold; caspase activator activity; receptor tyrosine kinase binding; protein phosphatase binding; ion channel inhibitor activity; receptor binding

Biological Process: caspase activation; negative regulation of phagocytosis; negative regulation of Wnt receptor signaling pathway; negative regulation of protein kinase B signaling cascade; viral reproduction; positive regulation of protein homooligomerization; regulation of cell cycle; positive regulation of apoptosis; rhythmic process; gastrulation; cell cycle; osteoblast differentiation; regulation of protein localization; positive regulation of mitochondrial depolarization; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of translation; positive regulation of cAMP catabolic process; positive regulation of cyclic-nucleotide phosphodiesterase activity; positive regulation of protein amino acid phosphorylation; negative regulation of cell growth; regulation of cell division; positive regulation of cell migration; positive regulation of GTPase activity