Glyceraldehyde 3-Phosphate Dehydrogenase, Rabbit, Western Blot Control Enzyme | GAPDH enzyme
Glyceraldehyde 3-Phosphate Dehydrogenase, Rabbit, Western Blot Control (GAPD, GAPDH, G3PD, MGC88685)
Purified
Purified
Dilution: Western Blot: Heat once and load 10ul/lane and visualize with G8140-14B. This preparation is intended for qualitative purpose and not to serve as standard of known concentration.
NCBI and Uniprot Product Information
Uniprot Description
Function: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation
By similarity.
Catalytic activity: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure: Homotetramer. Interacts with EIF1AD, USP25, PRKCI and WARS. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Interacts with CHP1; the interaction increases the binding of CHP1 with microtubules. Associates with microtubules. Component of the GAIT complex
By similarity. Ref.4
Subcellular location: Cytoplasm › cytosol
By similarity. Nucleus
By similarity. Cytoplasm › cytoskeleton
By similarity. Note: Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal
By similarity.
Post-translational modification: ISGylated
By similarity.S-nitrosylation of Cys-150 leads to interaction with SIAH1, followed by translocation to the nucleus
By similarity.Sulfhydration at Cys-150 increases catalytic activity
By similarity.
Sequence similarities: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
Research Articles on GAPDH
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Product Notes
The GAPDH gapdh (Catalog #AAA653888) is an Enzyme produced from Rabbit muscle and is intended for research purposes only. The product is available for immediate purchase. The Glyceraldehyde 3-Phosphate Dehydrogenase, Rabbit, Western Blot Control (GAPD, GAPDH, G3PD, MGC88685) reacts with Chicken, Frog, Human, Mouse, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's Glyceraldehyde 3-Phosphate Dehydrogenase, Rabbit, Western Blot Control can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Not suitable for ELISA or other applications where native protein is required. Dilution: Western Blot: Heat once and load 10ul/lane and visualize with G8140-14B. This preparation is intended for qualitative purpose and not to serve as standard of known concentration. Researchers should empirically determine the suitability of the GAPDH gapdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glyceraldehyde 3-Phosphate Dehydrogenase, Rabbit, Western Blot Control, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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