Rabbit anti-Human CYP4X1 Polyclonal Antibody | anti-CYP4X1 antibody

CYP4X1, NT (CYPIVX1, Cytochrome P450 4X1, UNQ1929/PRO4404) (APC)

Cat. Num. AAA6471746

• Gene Names: CYP4X1; CYPIVX1
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CYP4X1; Polyclonal Antibody; NT (CYPIVX1; Cytochrome P450 4X1; UNQ1929/PRO4404) (APC); anti-CYP4X1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CYP4X1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CYP4X1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human CYP4X1 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-CYP4X1 Antibody (N-term) in T47D cell line lysates (35ug/lane). CYP4X1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CYP4X1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-CYP4X1 antibody

Antibodies; Enzymes; Cytochrome

References

1. Savas, U., Arch. Biochem. Biophys. 436(2), 377-385 (2005). 2. Nelson, D.R., Pharmacogenetics 14(1), 1-18 (2004). 3. Bylund, J., Biochem. Biophys. Res. Commun. 296(3), 677-684 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 29837648
• NCBI GeneID: 260293
• NCBI Accession #: NP_828847
• NCBI GenBank Nucleotide #: NM_178033
• UniProt Accession #: Q8N118
• Molecular Weight: 59kDa
• NCBI Official Full Name: cytochrome P450 4X1 isoform 1
• NCBI Official Synonym Full Names: cytochrome P450 family 4 subfamily X member 1
• NCBI Official Symbol: CYP4X1
• NCBI Official Synonym Symbols: CYPIVX1
• NCBI Protein Information: cytochrome P450 4X1
• UniProt Protein Name: Cytochrome P450 4X1
• Protein Family: Cytochrome
• UniProt Gene Name: CYP4X1
• UniProt Entry Name: CP4X1_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes and is located within a cluster of genes belonging to this superfamily on chromosome 1. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The expression pattern of a similar rat protein suggests that this protein may be involved in neurovascular function in the brain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Uniprot Description

CYP4X1: a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The expression pattern of a similar rat protein suggests that this protein may be involved in neurovascular function in the brain. [provided by RefSeq, Jul 2008]

Protein type: EC 1.14.14.1; Oxidoreductase

Chromosomal Location of Human Ortholog: 1p33|1

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: iron ion binding; heme binding