Bovine Tumor Necrosis Factor Alpha (TNFa) RTU ELISA Kit | TNFa rtu elisa kit

Bovine Tumor Necrosis Factor Alpha (TNFa) ELISA Kit

Cat. Num. AAA4502967

• Gene Names: TNF; TNFa; TNF-a; TNF-alpha
• Reactivity: Bovine
• Synonyms: Tumor Necrosis Factor Alpha (TNFa); Bovine Tumor Necrosis Factor Alpha (TNFa) ELISA Kit; DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2; TNFa rtu elisa kit

• Reactivity: Bovine
• Specificity: This assay has high sensitivity and excellent specificity for detection of TNFa. No significant cross-reactivity or interference between TNFa and analogues was observed.
• Sequence Length: 234

• Samples: Bovine serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.1-5000pg/mL
• Sensitivity: <32pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TNFa were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TNFa were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TNFa rtu elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of TNFa in bovine serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNFa. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TNFa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNFa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TNFa in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 145279649
• NCBI GeneID: 280943
• NCBI Accession #: NP_776391.2
• NCBI GenBank Nucleotide #: NM_173966.3
• UniProt Accession #: Q06599; O18779; Q27978; A4IFT5; A9QWR7
• Molecular Weight: 25,439 Da
• NCBI Official Full Name: tumor necrosis factor
• NCBI Official Synonym Full Names: tumor necrosis factor
• NCBI Official Symbol: TNF
• NCBI Official Synonym Symbols: TNFa; TNF-a; TNF-alpha
• NCBI Protein Information: tumor necrosis factor
• UniProt Protein Name: Tumor necrosis factor
• UniProt Gene Name: TNF
• UniProt Synonym Gene Names: TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2

Uniprot Description

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.