Testing Data
(Image: CGRP terminal and fiber staining in rat dorsal horn (spinal cord), cryostat sections, ABC / DAB detection.)
Rabbit anti-Rat, Human CGRP Antibody | anti-CGRP antibody
CGRP, Antiserum
Tissue Preparation:
10um cryostats
- Fixative: 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4; 500 mL over ~20-30 min.
- Post Fixation: 1.5 hr. at 4°C in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4.
Immunofluorescence:
The antibody has significant fluorescein staining at a 1/100-1/200 dilution and significant Biotin-Streptavidin/HRP staining at a 1/2,000-1/4,000 dilution in rat amygdala, and spinal cord. The specificity of the antiserum was evaluated by soluble pre-adsorption with the peptides in question at a final concentration of 10-5 M. CGRP immunolabeling was completely abolished by pre-adsorption with rat α-CGRP and partially eliminated by pre-adsorption with rat β-CGRP. Pre-adsorption with the following peptides resulted in no loss of immunostaining: rat amylin, rat adrenomedulin, calcitonin, neurotensin, somatostatin, substance P, leucine enkephalin, methionine enkephalin, VIP, CCK-8, vasopressin and neuropeptide Y.
Dilutions listed as a recommendation. Optimal dilution should be determined by investigator.
Testing Data
(Image: CGRP terminal and fiber staining in rat dorsal horn (spinal cord), cryostat sections, ABC / DAB detection.)
Testing Data
(Image: CGRP terminal and fiber staining in rat dorsal horn (spinal cord), cryostat sections, ABC / DAB detection.)
Testing Data
Testing Data
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2014]
Uniprot Description
CALCA iso3: Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Belongs to the calcitonin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 11p15.2
Cellular Component: extracellular space; cell soma; cytoplasm; extracellular region; terminal button; nucleus
Molecular Function: identical protein binding; protein binding; calcitonin receptor binding; hormone activity; protein complex binding; receptor binding
Biological Process: negative regulation of osteoclast differentiation; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of interleukin-1 alpha production; vasodilation of artery during baroreceptor response to increased systemic arterial blood pressure; response to pain; positive regulation of vasodilation; G-protein signaling, adenylate cyclase activating pathway; protein amino acid phosphorylation; positive regulation of interleukin-8 production; monocyte chemotaxis; elevation of cytosolic calcium ion concentration; leukocyte adhesion; vasculature development; cell-cell signaling; neuropeptide signaling pathway; receptor internalization; negative regulation of smooth muscle contraction; regulation of blood pressure; positive regulation of cAMP biosynthetic process; negative regulation of ossification; feeding behavior; negative regulation of blood pressure; positive regulation of adenylate cyclase activity; negative regulation of bone resorption; inflammatory response; positive regulation of macrophage differentiation; detection of temperature stimulus involved in sensory perception of pain; negative regulation of neurological process; aging; cytosolic calcium ion homeostasis; regulation of heart rate; adenylate cyclase activation; positive regulation of ossification; regulation of systemic arterial blood pressure by neurological process; G-protein coupled receptor internalization; response to heat; endothelial cell proliferation; regulation of inflammatory response; activation of protein kinase activity; endothelial cell migration; negative regulation of transcription, DNA-dependent; embryo implantation
Research Articles on CGRP
Similar Products
Product Notes
The CGRP calca (Catalog #AAA555183) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CGRP, Antiserum reacts with Rat, Human and may cross-react with other species as described in the data sheet. Immunofluroescence: 1:100-1:200 (Fluorescein); 1:1,000-1:2,000 (Streptavidin/HRP) Tissue Preparation: 10um cryostats - Fixative: 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4; 500 mL over ~20-30 min. - Post Fixation: 1.5 hr. at 4°C in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4. Immunofluorescence: The antibody has significant fluorescein staining at a 1/100-1/200 dilution and significant Biotin-Streptavidin/HRP staining at a 1/2,000-1/4,000 dilution in rat amygdala, and spinal cord. The specificity of the antiserum was evaluated by soluble pre-adsorption with the peptides in question at a final concentration of 10-5 M. CGRP immunolabeling was completely abolished by pre-adsorption with rat α-CGRP and partially eliminated by pre-adsorption with rat β-CGRP. Pre-adsorption with the following peptides resulted in no loss of immunostaining: rat amylin, rat adrenomedulin, calcitonin, neurotensin, somatostatin, substance P, leucine enkephalin, methionine enkephalin, VIP, CCK-8, vasopressin and neuropeptide Y. Dilutions listed as a recommendation. Optimal dilution should be determined by investigator. Researchers should empirically determine the suitability of the CGRP calca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CGRP, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
