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Western Blot (WB) (Western Blot; Sample: Mouse Liver lysate;Primary Ab: 1µg/ml Rabbit Anti-Mouse DBI AntibodySecond Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: MBS2086047))

Rabbit Diazepam Binding Inhibitor (DBI) Antibody | anti-DBI antibody

Polyclonal Antibody to Diazepam Binding Inhibitor (DBI)

Gene Names
Dbi; EP; Acbp; ACBD1; endozepine
Reactivity
Mus musculus (Mouse)
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Diazepam Binding Inhibitor (DBI); Polyclonal Antibody to Diazepam Binding Inhibitor (DBI); anti-DBI antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mus musculus (Mouse)
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
0.69mg/ml (varies by lot)
Applicable Applications for anti-DBI antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Western blotting: 0.5-3ug/mL
Immunohistochemistry: 5-30ug/mL
Immunocytochemistry: 5-30ug/mL

Optimal working dilutions must be determined by end user.
Source
Polyclonal antibody preparation
Traits
Liquid
Cross Reactivity
Rat
Immunogen
Recombinant DBI (Ser2~Ile87) expressed in E.coli (MBS2010619).
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 24 months.

Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot; Sample: Mouse Liver lysate;Primary Ab: 1µg/ml Rabbit Anti-Mouse DBI AntibodySecond Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: MBS2086047))

Western Blot (WB) (Western Blot; Sample: Mouse Liver lysate;Primary Ab: 1µg/ml Rabbit Anti-Mouse DBI AntibodySecond Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: MBS2086047))

Western Blot (WB)

(Figure. Western Blot; Sample: Recombinant DBI, Mouse.)

Western Blot (WB) (Figure. Western Blot; Sample: Recombinant DBI, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P;Samples: Mouse Cerebellum Tissue;Primary Ab: 30ug/ml Rabbit Anti-MouseDBI AntibodySecond Ab: 2ug/mL HRP-LinkedCaprine Anti-Rabbit IgG PolyclonalAntibody(Catalog: MBS2086047))

Immunohistochemistry (IHC) (DAB staining on IHC-P;Samples: Mouse Cerebellum Tissue;Primary Ab: 30ug/ml Rabbit Anti-MouseDBI AntibodySecond Ab: 2ug/mL HRP-LinkedCaprine Anti-Rabbit IgG PolyclonalAntibody(Catalog: MBS2086047))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
acyl-CoA-binding protein isoform 2
NCBI Official Synonym Full Names
diazepam binding inhibitor
NCBI Official Symbol
Dbi
NCBI Official Synonym Symbols
EP; Acbp; ACBD1; endozepine
NCBI Protein Information
acyl-CoA-binding protein
UniProt Protein Name
Acyl-CoA-binding protein
Protein Family
UniProt Gene Name
Dbi
UniProt Synonym Gene Names
ACBP; DBI; EP

Uniprot Description

DBI: Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters. It is also able to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor. Belongs to the ACBP family. 6 isoforms of the human protein are produced by alternative promoter.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 1|1 E2.3

Cellular Component: contractile fiber; cytoplasm; endoplasmic reticulum; extracellular space; Golgi apparatus; mitochondrion; nucleus; plasma membrane; synaptic vesicle

Molecular Function: benzodiazepine receptor binding; fatty-acyl-CoA binding; lipid binding; protein dimerization activity

Biological Process: behavioral fear response; glial cell proliferation; hair follicle development; lateral ventricle development; learning and/or memory; negative regulation of fatty acid beta-oxidation; positive regulation of lipid biosynthetic process; positive regulation of release of sequestered calcium ion into cytosol; regulation of synaptic transmission, GABAergic; skin development; transport; triacylglycerol metabolic process

Research Articles on DBI

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Product Notes

The DBI dbi (Catalog #AAA2005542) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Diazepam Binding Inhibitor (DBI) reacts with Mus musculus (Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's Diazepam Binding Inhibitor (DBI) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). Western blotting: 0.5-3ug/mL Immunohistochemistry: 5-30ug/mL Immunocytochemistry: 5-30ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the DBI dbi for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Diazepam Binding Inhibitor (DBI), Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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