Diazepam Binding Inhibitor (DBI) Recombinant Protein | DBI recombinant protein

Recombinant Diazepam Binding Inhibitor (DBI)

Cat. Num. AAA2010619

• Gene Names: Dbi; EP; Acbp; ACBD1; endozepine
• Applications: SDS-Page, Western Blot
• Purity: > 95%
• Synonyms: Diazepam Binding Inhibitor (DBI); Recombinant Diazepam Binding Inhibitor (DBI); DBI recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Concentration: Original Concentration: 200 Ug/mL (varies by lot)
• Sequence: SQAEFDKAA EEVKRLKTQP TDEEMMLFIYS HFKQATVGDV NTDRPGLLDL KGKAKWDSWN KLKGTSKESA MKTYVEKVDE LKKKYGI

• Applicable Applications for DBI recombinant protein: Positive Control; Immunogen; SDS-PAGE; WB.; ; (May be suitable for use in other assays to be determined by the end user.)
• Organism Species: Mus musculus
• Source: Prokaryotic expression
• Residues: Ser2~Ile87
• Tags: N-terminal His Tag
• Traits: Freeze-dried powder
• Buffer: PBS, pH7.4, containing 0.01% SKL, 5% Trehalose and Proclin300.
• USAGE: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Subcellular Location: Endoplasmic reticulum lumen
• Predicted isoelectric point: 8.0
• Predicted Molecular Mass: 11.1kDa
• Accurate Molecular Mass: 13kDa as determined by SDS-PAGE reducing conditions.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles.; Store at 2-8ºC for one month.; Aliquot and store at -80ºC for 12 months.; ; Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6681137
• NCBI GeneID: 13167
• NCBI Accession #: NP_031856.1
• NCBI GenBank Nucleotide #: NM_007830.4
• UniProt Accession #: P31786
• NCBI Official Full Name: acyl-CoA-binding protein isoform 2
• NCBI Official Synonym Full Names: diazepam binding inhibitor
• NCBI Official Symbol: Dbi
• NCBI Official Synonym Symbols: EP; Acbp; ACBD1; endozepine
• NCBI Protein Information: acyl-CoA-binding protein; acyl-CoA binding protein; diazepam-binding inhibitor; diazepam binding inhibitor, splice form 1b
• UniProt Protein Name: Acyl-CoA-binding protein
• Protein Family: Acyl-CoA-binding protein
• UniProt Gene Name: Dbi
• UniProt Synonym Gene Names: ACBP; DBI; EP
• UniProt Entry Name: ACBP_MOUSE

Uniprot Description

DBI: Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters. It is also able to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor. Belongs to the ACBP family. 6 isoforms of the human protein are produced by alternative promoter.

Protein type: Lipid-binding

Cellular Component: Golgi apparatus; extracellular space; synaptic vesicle; contractile fiber; mitochondrion; endoplasmic reticulum; cytoplasm; plasma membrane; nucleus

Molecular Function: protein dimerization activity; benzodiazepine receptor binding; acyl-CoA binding; lipid binding

Biological Process: glial cell proliferation; skin development; behavioral fear response; triacylglycerol metabolic process; negative regulation of fatty acid beta-oxidation; learning and/or memory; hair follicle development; transport; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of lipid biosynthetic process; lateral ventricle development; regulation of synaptic transmission, GABAergic