Rabbit Smad5 Antibody | anti-Smad5 antibody

Smad5 Antibody

Cat. Num. AAA8503572

• Gene Names: SMAD5; DWFC; JV5-1; MADH5
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: Smad5; Smad5 Antibody; DKFZp781C1895; DKFZp781O1323; Dwfc; JV5-1; MADH5; anti-Smad5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 465

• Applicable Applications for anti-Smad5 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthetic peptide of human SMAD5
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Testing Data

(Western blot analysis of extracts of K562cell lines, using SMAD5 antibody.)

Related Product Information for anti-Smad5 antibody

Bone morphogenetic proteins (BMPs) constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation, and apoptosis (1, 2). BMP receptors are members of the TGF-beta family of Ser/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation, and activation of these receptors (3-5). They subsequently phosphorylate Smad1 at Ser463 and Ser465 in the carboxy-terminal motif SSXS, as well as Smad5 and Smad8 at their corresponding sites. These phosphorylated Smads dimerize with the coactivating Smad4 and translocate to the nucleus, where they stimulate transcription of target genes (5).MAP kinases and CDKs 8 and 9 phosphorylate residues in the linker region of Smad1, including Ser206. The phosphorylation of Ser206 recruits Smurf1 to the linker region and leads to the degradation of Smad1 (6). Phosphorylation of this site also promotes Smad1 transcriptional action by recruiting YAP to the linker region (7).

Product Categories/Family for anti-Smad5 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Hogan, B.L. et al. (1996) Genes Dev. 10, 1580-1594. 2. Hoodless, P.A. et al. (1996) Cell 85, 489-500. 3. Klemm, J.D. et al. (1998) Annu. Rev. Immunol. 16, 569-592. 4. Kretzschmar, M. et al. (1997) Genes Dev. 11, 984-995. 5. Whitman, M. (1998) Genes Dev. 12, 2445-2462. 6. Sapkota, G. et al. (2007) Mol Cell 25, 441-54. 7. Alarcón, C. et al. (2009) Cell 139, 757-69.

NCBI and Uniprot Product Information

• NCBI GI #: 47778929
• NCBI GeneID: 4090
• NCBI Accession #: NP_001001419.1
• NCBI GenBank Nucleotide #: NM_001001419.2
• UniProt Accession #: Q99717; O14688; Q15798; Q9UQA1
• Molecular Weight: 52kDa
• NCBI Official Full Name: mothers against decapentaplegic homolog 5
• NCBI Official Synonym Full Names: SMAD family member 5
• NCBI Official Symbol: SMAD5
• NCBI Official Synonym Symbols: DWFC; JV5-1; MADH5
• NCBI Protein Information: mothers against decapentaplegic homolog 5
• UniProt Protein Name: Mothers against decapentaplegic homolog 5
• Protein Family: SMAD5 antisense gene protein
• UniProt Gene Name: SMAD5
• UniProt Synonym Gene Names: MADH5; MAD homolog 5; Mothers against DPP homolog 5; SMAD 5; Smad5; hSmad5

NCBI Description

The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Uniprot Description

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).