Rabbit SMAD5 Polyclonal Antibody | anti-SMAD5 antibody

SMAD5 Antibody

Cat. Num. AAA9405325

• Gene Names: SMAD5; DWFC; JV5-1; MADH5
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: SMAD5; Polyclonal Antibody; SMAD5 Antibody; DKFZp781C1895; DKFZp781O1323; Dwfc; JV5-1; MADH5; anti-SMAD5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total SMAD5 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 465

• Applicable Applications for anti-SMAD5 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:100
• Immunogen Type: Peptide
• Immunogen Description: A synthetic peptide of human SMAD5.
• Target Name: SMAD5
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of K562 cell lines, using SMAD5 antibody.)

Related Product Information for anti-SMAD5 antibody

Bone morphogenetic proteins (BMPs) constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation, and apoptosis (1,2). BMP receptors are members of the TGF-b family of Ser/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation, and activation of these receptors (3-5). They subsequently phosphorylate Smad1 at Ser463 and Ser465 in the carboxy-terminal motif SSXS, as well as Smad5 and Smad8 at their corresponding sites. These phosphorylated Smads dimerize with the coactivating Smad4 and translocate to the nucleus, where they stimulate transcription of target genes (5).MAP kinases and CDKs 8 and 9 phosphorylate residues in the linker region of Smad1, including Ser206. The phosphorylation of Ser206 recruits Smurf1 to the linker region and leads to the degradation of Smad1 (6). Phosphorylation of this site also promotes Smad1 transcriptional action by recruiting YAP to the linker region (7).

Product Categories/Family for anti-SMAD5 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 47778929
• NCBI GeneID: 4090
• NCBI Accession #: NP_001001419.1
• NCBI GenBank Nucleotide #: NM_001001419.2
• UniProt Accession #: Q99717; O14688; Q15798; Q9UQA1
• Molecular Weight: 52,258 Da
• NCBI Official Full Name: mothers against decapentaplegic homolog 5
• NCBI Official Synonym Full Names: SMAD family member 5
• NCBI Official Symbol: SMAD5
• NCBI Official Synonym Symbols: DWFC; JV5-1; MADH5
• NCBI Protein Information: mothers against decapentaplegic homolog 5
• UniProt Protein Name: Mothers against decapentaplegic homolog 5
• Protein Family: SMAD5 antisense gene protein
• UniProt Gene Name: SMAD5
• UniProt Synonym Gene Names: MADH5; MAD homolog 5; Mothers against DPP homolog 5; SMAD 5; Smad5; hSmad5
• UniProt Entry Name: SMAD5_HUMAN

NCBI Description

The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Uniprot Description

SMAD5: transcription factor phosphorylated and activated by bone morphogenetic protein receptor kinases. Phosphorylated Smads dimerize with collaborating Smad4 and are translocated into the nucleus, where they regulate transcription of target genes. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis.

Protein type: DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: nucleoplasm; transcription factor complex; protein complex; cytoplasm; integral to membrane; intracellular; nucleus; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding; metal ion binding; receptor signaling protein activity; transcription factor activity; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity

Biological Process: Mullerian duct regression; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; signal transduction; embryonic pattern specification; protein amino acid phosphorylation; germ cell development; BMP signaling pathway; positive regulation of osteoblast differentiation; ureteric bud development; transforming growth factor beta receptor signaling pathway; cartilage development; erythrocyte differentiation; cardiac muscle contraction; osteoblast fate commitment