Human Prosaposin (PSAP) RTU ELISA Kit | PSAP rtu elisa kit

Human Prosaposin (PSAP) ELISA Kit

Cat. Num. AAA4502063

• Gene Names: PSAP; GLBA; SAP1
• Reactivity: Human
• Synonyms: Prosaposin (PSAP); Human Prosaposin (PSAP) ELISA Kit; SAP1; GLBA; CSAct; Dispersin; Pro-Saposin; Proactivator polypeptide; Glucosylceramidase activator; Sulfatide/GM1 activator; Cerebroside sulfate activator; Sphingolipid Activator 1; PSAP rtu elisa kit

• Reactivity: Human
• Sequence Length: 527

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cerebrospinal Fluid, Seminal Plasma, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.055ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PSAP rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PSAP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PSAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PSAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PSAP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 110224476
• NCBI GeneID: 5660
• NCBI Accession #: NP_001035930.1
• NCBI GenBank Nucleotide #: NM_001042465.2
• UniProt Accession #: P07602; P07292; P15793; P78538; P78541; P78546; P78547; P78558; Q53Y86; Q6IBQ6; Q92739; Q92740
• Molecular Weight: 58,484 Da
• NCBI Official Full Name: prosaposin isoform b preproprotein
• NCBI Official Synonym Full Names: prosaposin
• NCBI Official Symbol: PSAP
• NCBI Official Synonym Symbols: GLBA; SAP1
• NCBI Protein Information: prosaposin
• UniProt Protein Name: Prosaposin
• Protein Family: Prosaposin
• UniProt Gene Name: PSAP
• UniProt Synonym Gene Names: GLBA; SAP1; CSAct; SAP-1; SAP-2

NCBI Description

This gene encodes a highly conserved preproprotein that is proteolytically processed to generate four main cleavage products including saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]

Uniprot Description

Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate.Saposin-B stimulates the hydrolysis of galacto-cerebroside sulfate by arylsulfatase A (EC 3.1.6.8), GM1 gangliosides by beta-galactosidase (EC 3.2.1.23) and globotriaosylceramide by alpha-galactosidase A (EC 3.2.1.22). Saposin-B forms a solubilizing complex with the substrates of the sphingolipid hydrolases.Saposin-D is a specific sphingomyelin phosphodiesterase activator (EC 3.1.4.12).Prosaposin: Behaves as a myelinotrophic and neurotrophic factor, these effects are mediated by its G-protein-coupled receptors, GPR37 and GPR37L1, undergoing ligand-mediated internalization followed by ERK phosphorylation signaling.