Mouse Neurofilament (NF-H) Monoclonal Antibody | anti-NF-H antibody

Neurofilament (NF-H) (Neuronal Marker)

Cat. Num. AAA4380134

• Gene Names: NEFH; NFH; CMT2CC
• Reactivity: Human, Mouse, Rat, Pig, Chicken. Others not known.
• Applications: Immunohistochemistry
• Synonyms: Neurofilament (NF-H); Monoclonal Antibody; Neurofilament (NF-H) (Neuronal Marker); NEFH; Neurofilament H; Neurofilament Heavy Polypeptide 200kDa; Neurofilament Triplet H Protein; NF-H; NF200; anti-NF-H antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Pig, Chicken. Others not known.
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: rNF421
• Specificity: This MAb reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 1020

• Applicable Applications for anti-NF-H antibody: Immunohistochemistry (IHC) Formalin
• Application Notes: IHC-F: 0.5-1.0ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
• Immunogen: Recombinant human neurofilament protein
• Cellular Localization: Cytoplasmic
• Hu-chromosome Location: 22q12.2
• Positive Control: Brain, Neuroblastoma
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Brain stained with Neurofilament Mouse Recombinant Monoclonal Antibody (rNF421).)

SDS-Page

(SDS-PAGE Analysis of Purified Neurofilament Mouse Recombinant Monoclonal Antibody (rNF421).)

References

Shaw G et. al. European Journal of Cell Biology. 34: 130-136 (1984). Shaw G et. al. European Journal of Cell Biology. 42: 1-9 (1986).

NCBI and Uniprot Product Information

• NCBI GI #: 32483416
• NCBI GeneID: 4744
• NCBI Accession #: NP_066554.2
• NCBI GenBank Nucleotide #: NM_021076.3
• UniProt Accession #: P12036; Q96HF8; Q9UJS7; Q9UQ14; B4DYY4
• Molecular Weight: 200kDa
• NCBI Official Full Name: neurofilament heavy polypeptide
• NCBI Official Synonym Full Names: neurofilament heavy
• NCBI Official Symbol: NEFH
• NCBI Official Synonym Symbols: NFH; CMT2CC
• NCBI Protein Information: neurofilament heavy polypeptide
• UniProt Protein Name: Neurofilament heavy polypeptide
• UniProt Gene Name: NEFH
• UniProt Synonym Gene Names: KIAA0845; NFH; NF-H

NCBI Description

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.