Western Blot (WB)
(Western blot analysis of CDC6 using RAW264.7 whole lysates)
Rabbit anti-Human CDC6 Polyclonal Antibody | anti-CDC6 antibody
CDC6 Antibody
Gene Names
CDC6; CDC18L; HsCDC6; MGORS5; HsCDC18
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CDC6; Polyclonal Antibody; CDC6 Antibody; Cdc 18L; Cdc 6; CDC18 (cell division cycle 18; S.pombe; homolog) like; CDC18 (S.pombe); Cdc18 related protein; CDC18(S.pombe); Cdc18-related protein; Cdc18L; cdc6; CDC6 cell division cycle 6 homolog; CDC6 related protein; CDC6-related protein; CDC6_HUMAN; Cdc6p; CELL CYCLE CONTROLLER CDC6; Cell division control protein 6; Cell division control protein 6 homolog; Cell division cycle 6 homolog (S. cerevisiae); Cell division cycle 6 homolog; Cell division cycle 6; S. cerevisiae; homolog of; HsCDC 6; HsCDC18; HsCDC6; p62; p62(cdc 6); p62(cdc6); anti-CDC6 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
CDC6 antibody detects endogenous levels of total CDC6
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
560
Applicable Applications for anti-CDC6 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human CDC6
Subcellular Location
Nucleus. Cytoplasm. The protein is nuclear in G1 and cytoplasmic in S-phase cells.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (91%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of CDC6 using RAW264.7 whole lysates)
Western Blot (WB)
(Western blot analysis of CDC6 using RAW264.7 whole lysates)
Immunofluorescene (IF)
(MBS9608382 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescene (IF)
(MBS9608382 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-CDC6 antibody
Function: Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
Subunit Structure: Interacts with PCNA, ORC1, cyclin-CDK (PubMed:9566895). Interacts with HUWE1 (PubMed:17567951). Interacts with ANKRD17 (PubMed:23711367). Interacts with GRWD1; origin binding of GRWD1 is dependent on CDC6 (PubMed:25990725). Interacts with CDT1; are mutually dependent on one another for loading MCM complexes onto chromatin (PubMed:14672932).
Similarity: Belongs to the CDC6/cdc18 family.
Subunit Structure: Interacts with PCNA, ORC1, cyclin-CDK (PubMed:9566895). Interacts with HUWE1 (PubMed:17567951). Interacts with ANKRD17 (PubMed:23711367). Interacts with GRWD1; origin binding of GRWD1 is dependent on CDC6 (PubMed:25990725). Interacts with CDT1; are mutually dependent on one another for loading MCM complexes onto chromatin (PubMed:14672932).
Similarity: Belongs to the CDC6/cdc18 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 63 kDa
Predicted: 63 kDa
Predicted: 63 kDa
NCBI Official Full Name
cell division control protein 6 homolog
NCBI Official Synonym Full Names
cell division cycle 6
NCBI Official Symbol
CDC6
NCBI Official Synonym Symbols
CDC18L; HsCDC6; MGORS5; HsCDC18
NCBI Protein Information
cell division control protein 6 homolog
UniProt Protein Name
Cell division control protein 6 homolog
Protein Family
UniProt Gene Name
CDC6
UniProt Synonym Gene Names
CDC18L; HsCdc18; HsCDC6
NCBI Description
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq, Jul 2008]
Uniprot Description
Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
Research Articles on CDC6
Similar Products
Product Notes
The CDC6 cdc6 (Catalog #AAA9608382) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDC6 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CDC6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:1000-3000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the CDC6 cdc6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDC6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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