Rabbit Jagged 1 Polyclonal Antibody | anti-JAG1 antibody

Anti-Jagged 1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101812

• Gene Names: Jag1; Htu; Ozz; ABE2; Ser-1; Gsfabe2
• Reactivity: Mouse; Predicted to React with: Human, Rat (Species predicted to react based on 100% sequence homology)
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: Jagged 1; Polyclonal Antibody; Anti-Jagged 1 Antibody; Rabbit Polyclonal; Jagged 1/Jag1 Antibody; Rabbit PAb; Antigen Affinity Purified; jagged 1; Anti-ABE2 Antibody; Anti-Gsfabe2 Antibody; Anti-Htu Antibody; Anti-Ozz Antibody; Anti-Ser-1 Antibody; anti-JAG1 antibody

• Host: Rabbit
• Reactivity: Mouse; Predicted to React with: Human, Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse Jagged 1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-JAG1 antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:100-1:400; ICC/IF: 1:100-1:500
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Mouse Jagged 1/Jag1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Mouse Jagged 1/Jag1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of mouse Jag1 in mouse kidney with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of Jag1 in RAW264.7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Mouse Jag1 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm and cell membrane.)

Related Product Information for anti-JAG1 antibody

Protein Jagged 1, also known as JAG1, JAGL1, and CD339, is a single-pass type I membrane protein that contains 1 DSL domain and 15 EGF-like domains. JAG1/Jagged 1 is widely expressed in adult and fetal tissues. The expression of JAG1/Jagged 1 is up-regulated in cervical squamous cell carcinoma. JAG1/Jagged 1 is also expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells. JAG1/Jagged 1 is a ligand for multiple Notch receptors. It is involved in the mediation of Notch signaling. JAG1/Jagged 1 may be involved in cell-fate decisions during hematopoiesis. JAG1/Jagged 1 seems to be involved in the early and late stages of mammalian cardiovascular development. It inhibits myoblast differentiation and enhances fibroblast growth factor-induced angiogenesis. Defects in JAG1/Jagged 1 are the cause of Alagille syndrome type 1 (ALGS1). Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. Defects in JAG1/Jagged 1 are also a cause of tetralogy of Fallot (TOF). TOF is a congenital heart anomaly that consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left), and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation.

Product Categories/Family for anti-JAG1 antibody

Notch Pathway

References

Oda T.et al., 1997, Nat. Genet. 16:235-242.Krantz I.D. et al., 1998, Am. J. Hum. Genet. 62:1361-1369.Li L. et al., 1998, Immunity. 8:43-55.Jones E.A. et al., 2000, J. Med. Genet. 37: 658-662.Roepke A.et al., 2003, Hum. Mutat. 21:100-100.Jurkiewicz D.et al., 2005, Hum. Mutat. 25:321-321.Warthen D.M.et al., 2006, Hum. Mutat. 27:436-443.

NCBI and Uniprot Product Information

• NCBI GI #: 7305197
• NCBI GeneID: 16449
• NCBI Accession #: NP_038850.1
• NCBI GenBank Nucleotide #: NM_013822.5
• UniProt Accession #: Q9QXX0
• Molecular Weight: 134,164 Da
• NCBI Official Full Name: protein jagged-1
• NCBI Official Synonym Full Names: jagged 1
• NCBI Official Symbol: Jag1
• NCBI Official Synonym Symbols: Htu; Ozz; ABE2; Ser-1; Gsfabe2
• NCBI Protein Information: protein jagged-1
• UniProt Protein Name: Protein jagged-1
• Protein Family: Protein jagged
• UniProt Gene Name: Jag1
• UniProt Synonym Gene Names: Jagged1

Uniprot Description

Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (). May regulate fibroblast growth factor-induced angiogenesis.