Human Hif1 Alpha ELISA Kit | Hif1 Alpha elisa kit

Human Hif1 Alpha Pre Coated ELISA Kit

Cat. Num. AAA669190

• Gene Names: HIF1A; HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
• Reactivity: Human
• Applications: ELISA
• Synonyms: Hif1 Alpha; Human Hif1 Alpha Pre Coated ELISA Kit; Hif1 Alpha elisa kit

• Reactivity: Human
• Sequence Length: 826

• Applicable Applications for Hif1 Alpha elisa kit: ELISA (EIA)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Hif1 Alpha elisa kit

Background/Introduction: Hypoxia inducible factor-1 (HIF) is a heterodimeric protein including an oxygen-regulated alpha subunit and a constitutive expressed beta subunit. Hypoxia inducible factor-1 alpha (HIF- 1alpha) is a master regulator of cellular adaptation to oxygen deprivation and activates transcription of genes involved in tumor metabolism, angiogenesis, invasion and metastasis, all of which are implicated in cancer progression. Several domains of HIF-1alpha mediate protein-protein interaction, which is essential for the formation of the active heterodimer with HIF-1beta. Targeting specific domains of HIF-1alpha might lead to the identification of more selective inhibitors. Alpha subunits are degraded during normoxia mainly through a proteasomedependent pathway after hydroxylation of two proline residues by prolyl-hydroxilases (PHDs). During hypoxia, PHDs are inhibited and HIF-1alpha subunit accumulates, dimerizes with HIF-1beta and drive expression of HIF target genes which include genes involved in angiogenesis and tissue repair such as vascular endothelial growth factor (VEGF) or erythropoietin (EPO) and prolilhydroxilases (PHDs) genes, among others.

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NCBI and Uniprot Product Information

• NCBI GI #: 15214788
• NCBI GeneID: 3091
• Molecular Weight: 95,634 Da
• NCBI Official Full Name: Hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
• NCBI Official Synonym Full Names: hypoxia inducible factor 1 alpha subunit
• NCBI Official Symbol: HIF1A
• NCBI Official Synonym Symbols: HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
• NCBI Protein Information: hypoxia-inducible factor 1-alpha
• UniProt Protein Name: Hypoxia-inducible factor 1-alpha
• UniProt Gene Name: HIF1A
• UniProt Synonym Gene Names: bHLHe78

NCBI Description

This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011]

Uniprot Description

Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.