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Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using 212213 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug/lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Kit)
Rabbit NDRG1 Polyclonal Antibody | anti-NDRG1 antibody
NDRG1 (N-myc Downstream Regulated Gene 1, CAP43, CMT4D, DRG1, GC4, HMSNL, NDR1, NMSL, PROXY1, RIT42, RTP, TARG1, TDD5) (Biotin)
Gene Names
NDRG1; GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Western Blot, Immunohistochemistry
Purity
Purified by affinity chromatography.
Synonyms
NDRG1; Polyclonal Antibody; NDRG1 (N-myc Downstream Regulated Gene 1; CAP43; CMT4D; DRG1; GC4; HMSNL; NDR1; NMSL; PROXY1; RIT42; RTP; TARG1; TDD5) (Biotin); anti-NDRG1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes NDRG1. Species Crossreactivity: human, mouse, rat
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Concentration
~1mg/ml (varies by lot)
Applicable Applications for anti-NDRG1 antibody
Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
IF: 1:50-1:200
WB: 1:500-1:2000
IHC: 1:50-1:200
Applications are based on unconjugated antibody.
WB: 1:500-1:2000
IHC: 1:50-1:200
Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to human NDRG1.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using 212213 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug/lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Kit)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using 212213 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug/lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Kit)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cells using 212213. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cells using 212213. Blue: DAPI for nuclear staining.)
Testing Data
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Product Categories/Family for anti-NDRG1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
394
NCBI Official Full Name
protein NDRG1 isoform 1
NCBI Official Synonym Full Names
N-myc downstream regulated 1
NCBI Official Symbol
NDRG1
NCBI Official Synonym Symbols
GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1
NCBI Protein Information
protein NDRG1; protein regulated by oxygen-1; differentiation-related gene 1 protein; nickel-specific induction protein Cap43; N-myc downstream-regulated gene 1 protein; reducing agents and tunicamycin-responsive protein
UniProt Protein Name
Protein NDRG1
Protein Family
UniProt Gene Name
NDRG1
UniProt Synonym Gene Names
CAP43; DRG1; RTP; DRG-1; RTP
UniProt Entry Name
NDRG1_HUMAN
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Product Notes
The NDRG1 ndrg1 (Catalog #AAA6428467) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NDRG1 (N-myc Downstream Regulated Gene 1, CAP43, CMT4D, DRG1, GC4, HMSNL, NDR1, NMSL, PROXY1, RIT42, RTP, TARG1, TDD5) (Biotin) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NDRG1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC). IF: 1:50-1:200 WB: 1:500-1:2000 IHC: 1:50-1:200 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NDRG1 ndrg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDRG1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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