Rabbit ARNT Antibody | anti-ARNT antibody

ARNT Antibody

Cat. Num. AAA8502633

• Gene Names: ARNT; HIF1B; TANGO; bHLHe2; HIF1BETA; HIF-1beta; HIF1-beta; HIF-1-beta
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: ARNT; ARNT Antibody; HIF-1beta; HIF1B; HIF1BETA; TANGO; bHLHe2; anti-ARNT antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 773

• Applicable Applications for anti-ARNT antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human ARNT
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using ARNT antibody.)

Related Product Information for anti-ARNT antibody

Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1). The HIF1 complex consists of two subunits, HIF-1alpha and HIF-1beta, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1alpha is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1alpha is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3, 4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1alpha degradation and lead to its stabilization. In addition, HIF-1alpha can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).HIF-1beta is also known as AhR nuclear translocator (ARNT) due to its ability to partner with the aryl hydrocarbon receptor (AhR) to form a heterodimeric transcription factor complex (8). Together with AhR, HIF-1beta plays an important role in xenobiotics metabolism (8). In addition, a chromosomal translocation leading to a TEL-ARNT fusion protein is associated with acute myeloblastic leukemia (9). Studies also found that ARNT/HIF-1beta expression levels decrease significantly in pancreatic islets from patients with type 2 diabetes, suggesting that HIF-1beta plays an important role in pancreatic beta-cell function (10).

Product Categories/Family for anti-ARNT antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Sharp, F.R. and Bernaudin, M. (2004) Nat Rev Neurosci 5, 437-48. 2. Wang, G.L. et al. (1995) Proc Natl Acad Sci U S A 92, 5510-4. 3. Jaakkola, P. et al. (2001) Science 292, 468-72. 4. Maxwell, P.H. et al. (1999) Nature 399, 271-5. 5. Fukuda, R. et al. (2002) J Biol Chem 277, 38205-11. 6. Jiang, B.H. et al. (2001) Cell Growth Differ 12, 363-9. 7. Laughner, E. et al. (2001) Mol Cell Biol 21, 3995-4004. 8. Walisser, J.A. et al. (2004) Proc Natl Acad Sci U S A 101, 16677-82. 9. Salomon-Nguyen, F. et al. (2000) Proc Natl Acad Sci U S A 97, 6757-62. 10. Gunton, J.E. et al. (2005) Cell 122, 337-49.

NCBI and Uniprot Product Information

• NCBI GI #: 309747071
• NCBI GeneID: 405
• NCBI Accession #: NP_001184254.1
• NCBI GenBank Nucleotide #: NM_001197325.1
• UniProt Accession #: P27540; Q59ED4; Q5QP39; Q8NDC7; B2R9H1; C4AMA1; F8WAP6
• Molecular Weight: 87kDa
• NCBI Official Full Name: aryl hydrocarbon receptor nuclear translocator isoform 4
• NCBI Official Synonym Full Names: aryl hydrocarbon receptor nuclear translocator
• NCBI Official Symbol: ARNT
• NCBI Official Synonym Symbols: HIF1B; TANGO; bHLHe2; HIF1BETA; HIF-1beta; HIF1-beta; HIF-1-beta
• NCBI Protein Information: aryl hydrocarbon receptor nuclear translocator
• UniProt Protein Name: Aryl hydrocarbon receptor nuclear translocator
• Protein Family: Aryl hydrocarbon receptor nuclear translocator
• UniProt Gene Name: ARNT
• UniProt Synonym Gene Names: BHLHE2; ARNT protein; bHLHe2; HIF-1-beta; HIF1-beta

NCBI Description

This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013]

Uniprot Description

Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.