Aryl hydrocarbon receptor nuclear translocator (ARNT) Recombinant Protein | ARNT recombinant protein

Recombinant Human Aryl hydrocarbon receptor nuclear translocator (ARNT), partial

Cat. Num. AAA968128

• Gene Names: ARNT; HIF1B; TANGO; bHLHe2; HIF1BETA; HIF-1beta; HIF1-beta; HIF-1-beta
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Aryl hydrocarbon receptor nuclear translocator (ARNT); Recombinant Human Aryl hydrocarbon receptor nuclear translocator (ARNT); partial; ARNT recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-474. partial
• Sequence: MAATTANPEMTSDVPSLGPAIASGNSGPGIQGGGAIVQRAIKRRPGLDFDDDGEGNSKFLRCDDDQMSNDKERFARSDDEQSSADKERLARENHSEIERRRRNKMTAYITELSDMVPTCSALARKPDKLTILRMAVSHMKSLRGTGNTSTDGSYKPSFLTDQELKHLILEAADGFLFIVSCETGRVVYVSDSVTPVLNQPQSEWFGSTLYDQVHPDDVDKLREQLSTSENALTGRILDLKTGTVKKEGQQSSMRMCMGSRRSFICRMRCGSSSVDPVSVNRLSFVRNRCRNGLGSVKDGEPHFVVVHCTGYIKAWPPAGVSLPDDDPEAGQGSKFCLVAIGRLQVTSSPNCTDMSNVCQPTEFISRHNIEGIFTFVDHRCVATVGYQPQELLGKNIVEFCHPEDQQLLRDSFQQVVKLKGQVLSVMFRFRSKNQEWLWMRTSSFTFQNPYSDEIEYIICTNTNVKNSSQEPRPT

• Species: Human
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

SDS-Page

Related Product Information for ARNT recombinant protein

The aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. This gene encodes a protein that forms a complex with the ligand-bound Ah receptor, and is required for receptor function. The encoded protein has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1 (HIF1). A t(1;12)(q21;p13) translocation, which results in a TEL-ARNT fusion protein, is associated with acute myeloblastic leukemia. Three alternatively spliced variants encoding different isoforms have been described for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 309747071
• NCBI GeneID: 405
• NCBI Accession #: NP_001184254.1
• NCBI GenBank Nucleotide #: NM_001197325.1
• UniProt Accession #: P27540; Q59ED4; Q5QP39; Q8NDC7; B2R9H1; C4AMA1; F8WAP6
• Molecular Weight: 54.8 kDa
• NCBI Official Full Name: aryl hydrocarbon receptor nuclear translocator isoform 4
• NCBI Official Synonym Full Names: aryl hydrocarbon receptor nuclear translocator
• NCBI Official Symbol: ARNT
• NCBI Official Synonym Symbols: HIF1B; TANGO; bHLHe2; HIF1BETA; HIF-1beta; HIF1-beta; HIF-1-beta
• NCBI Protein Information: aryl hydrocarbon receptor nuclear translocator
• UniProt Protein Name: Aryl hydrocarbon receptor nuclear translocator
• Protein Family: Aryl hydrocarbon receptor nuclear translocator
• UniProt Gene Name: ARNT
• UniProt Synonym Gene Names: BHLHE2; ARNT protein; bHLHe2; HIF-1-beta; HIF1-beta

NCBI Description

This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013]

Uniprot Description

Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.