Human Angiopoietin-2 ELISA Kit | ANGPT2 elisa kit

Human Angiopoietin-2 ELISA Kit

Cat. Num. AAA824627

• Gene Names: ANGPT2; ANG2; AGPT2
• Reactivity: Human
• Applications: ELISA
• Synonyms: Angiopoietin-2; Human Angiopoietin-2 ELISA Kit; ANG-2; ANGPT2 elisa kit

• Reactivity: Human
• Specificity: The Rat TGF beta 1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat TGF beta 1 proteins within the range of 62.5 pg/ml-4000 pg/ml.
• Sequence Length: 495

• Applicable Applications for ANGPT2 elisa kit: Sandwich ELISA (SE)
• Samples: Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates
• Sensitivity: 30 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ANGPT2 elisa kit

Principle of the Assay: MBS Rat TGF beta 1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat TGF beta 1 in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Rat TGF beta 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TGF beta 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat TGF beta 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TGF beta 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm!!Background/Introduction: Transforming growth factor-beta 1 (TGF-beta 1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects. TGF beta 1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation. TGF beta 1 is also an important mediator of bone remodeling. TGF beta 1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation. TGF-beta 1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta 1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion. TGF beta 1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide (beta 1-LAP) in either the presence or the absence of additional latent TGF-beta 1-binding protein.

NCBI and Uniprot Product Information

• NCBI GI #: 169646749
• NCBI GeneID: 285
• NCBI Accession #: NP_001112359.1
• NCBI GenBank Nucleotide #: NM_001118887.1
• UniProt Accession #: O15123; Q9NRR7; Q9P2Y7; A0AV38; A8K205; B7ZLM7
• Molecular Weight: 56,848 Da
• NCBI Official Full Name: angiopoietin-2 isoform b
• NCBI Official Synonym Full Names: angiopoietin 2
• NCBI Official Symbol: ANGPT2
• NCBI Official Synonym Symbols: ANG2; AGPT2
• NCBI Protein Information: angiopoietin-2; ANG-2; Tie2-ligand; angiopoietin-2B; angiopoietin-2a
• UniProt Protein Name: Angiopoietin-2
• Protein Family: Angiopoietin
• UniProt Gene Name: ANGPT2
• UniProt Synonym Gene Names: ANG-2
• UniProt Entry Name: ANGP2_HUMAN

NCBI Description

The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ANGPT2: Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. Interacts with TEK/TIE2, competing for the same binding site as ANGPT1. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: extracellular space; cell projection; plasma membrane; extracellular region; nucleus

Molecular Function: protein binding; metal ion binding; receptor tyrosine kinase binding; receptor binding

Biological Process: Tie receptor signaling pathway; organ regeneration; maternal process involved in pregnancy; negative regulation of blood vessel endothelial cell migration; signal transduction; response to organic cyclic substance; germ cell development; negative regulation of positive chemotaxis; positive regulation of angiogenesis; negative regulation of angiogenesis; response to radiation; response to mechanical stimulus; response to hypoxia; response to glucose stimulus; response to activity; angiogenesis; blood coagulation; leukocyte migration