Rabbit anti-Human, mouse ANGPT2 Polyclonal Antibody | anti-ANGPT2 antibody

ANGPT2 Antibody (C-term)

Cat. Num. AAA9204486

• Gene Names: ANGPT2; ANG2; AGPT2
• Reactivity: Human, mouse
• Applications: ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: ANGPT2; Polyclonal Antibody; ANGPT2 Antibody (C-term); Angiopoietin-2; ANG-2; anti-ANGPT2 antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This ANGPT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 404-432 amino acids from the C-terminal region of human ANGPT2.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 404-432
• Sequence Length: 495

• Applicable Applications for anti-ANGPT2 antibody: ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB)
• Application Notes: IHC~~1:50~100; FC~~1:10~50; WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Immunohistochemistry (IHC)

(ANGPT2 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the ANGPT2 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ANGPT2 Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(ANGPT2 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the ANGPT2 antibody detected the ANGPT2 protein (arrow).)

Western Blot (WB)

(ANGPT2 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the ANGPT2 antibody detected the ANGPT2 protein (arrow).)

Related Product Information for anti-ANGPT2 antibody

The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene.

Product Categories/Family for anti-ANGPT2 antibody

Cardiovascular; Developmental Biology

References

Morrissey, C., et al. Prostate 70(16):1799-1808(2010) Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) : Bento, C.F., et al. Exp. Physiol. 95(9):955-970(2010) Vrbacky, F., et al. Hematology 15(4):210-214(2010) Chen, J., et al. Biochem. Biophys. Res. Commun. 398(2):212-216(2010)

NCBI and Uniprot Product Information

• NCBI GI #: 169646749
• NCBI GeneID: 285
• NCBI Accession #: NP_001112359.1; NP_001112360.1; NP_001138.1
• NCBI GenBank Nucleotide #: NM_001118887.1
• UniProt Accession #: O15123; Q9NRR7; Q9P2Y7; A0AV38; A8K205; B7ZLM7
• Molecular Weight: 56919
• NCBI Official Full Name: angiopoietin-2 isoform b
• NCBI Official Synonym Full Names: angiopoietin 2
• NCBI Official Symbol: ANGPT2
• NCBI Official Synonym Symbols: ANG2; AGPT2
• NCBI Protein Information: angiopoietin-2
• UniProt Protein Name: Angiopoietin-2
• Protein Family: Angiopoietin
• UniProt Gene Name: ANGPT2
• UniProt Synonym Gene Names: ANG-2
• UniProt Entry Name: ANGP2_HUMAN

NCBI Description

The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ANGPT2: Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. Interacts with TEK/TIE2, competing for the same binding site as ANGPT1. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: extracellular space; cell projection; extracellular region; plasma membrane; nucleus

Molecular Function: protein binding; metal ion binding; receptor tyrosine kinase binding; receptor binding

Biological Process: Tie receptor signaling pathway; organ regeneration; maternal process involved in pregnancy; signal transduction; negative regulation of blood vessel endothelial cell migration; response to organic cyclic substance; germ cell development; negative regulation of positive chemotaxis; positive regulation of angiogenesis; response to radiation; negative regulation of angiogenesis; response to mechanical stimulus; response to hypoxia; response to glucose stimulus; response to activity; angiogenesis; blood coagulation; leukocyte migration