Western Blot (WB)
(Western blot of Acetyl-Lysine antibody (rAb). HeLa cells nontreated or treated with Trichostatin A (TSA), were probed using Acetyl-Lysine antibody (RM101) at 0.5 ug/ml.)
Rabbit anti-Human Acetyl-Lysine Antibody | anti-Acetyl-Lysine antibody
Acetyl-Lysine antibody (rAb)(Clone RM101)
Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Purity
Protein A Chromatography
Synonyms
Acetyl-Lysine; Acetyl-Lysine antibody (rAb)(Clone RM101); anti-Acetyl-Lysine antibody
Host
Rabbit
Reactivity
Human
Isotype
IgG
Clone Number
RM101
Purity/Purification
Protein A Chromatography
Form/Format
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-Acetyl-Lysine antibody
Immunocytochemistry (ICC), Western Blot (WB)
Application Notes
Applications Validated:
WB: 0.5-2ug/ml
ICC: 2-10ug/ml
WB: 0.5-2ug/ml
ICC: 2-10ug/ml
Antibody Type
Recombinant Antibody
Immunogen
Acetyllysine-BSA
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(Western blot of Acetyl-Lysine antibody (rAb). HeLa cells nontreated or treated with Trichostatin A (TSA), were probed using Acetyl-Lysine antibody (RM101) at 0.5 ug/ml.)
Western Blot (WB)
(Western blot of Acetyl-Lysine antibody (rAb). HeLa cells nontreated or treated with Trichostatin A (TSA), were probed using Acetyl-Lysine antibody (RM101) at 0.5 ug/ml.)
Immunofluorescence (IF)
(Immunofluorescence stain of Acetyl-Lysine antibody (rAb). HeLa cells were stained using Acetyl-Lysine antibody (RM101) at 2 ug/ml (red). Actin filaments was labeled with fluorescein phalloidin (green), and nucleus stained with DAPI (blue).)
Immunofluorescence (IF)
(Immunofluorescence stain of Acetyl-Lysine antibody (rAb). HeLa cells were stained using Acetyl-Lysine antibody (RM101) at 2 ug/ml (red). Actin filaments was labeled with fluorescein phalloidin (green), and nucleus stained with DAPI (blue).)
Related Product Information for anti-Acetyl-Lysine antibody
Short Description: Our Acetyl-Lysine antibody (rAb)(Clone RM101) was raised in a Rabbit host. It has been validated for use in Immunocytochemistry and Western blot; it has been shown to react with Human samples, but the sequence is not species specific so it should react with a wide range of sample types.
Background: Lysine N-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Acetylation of histones can occur at several different lysine positions in the histone tail, and is performed by Histone Acetyltransferases (HATs) such as HAT1 or GCN5. Acetylation of histones is often associated with transcriptional activation. Clone RM101 reacts to lysine-acetylated proteins. No cross reactivity with nonacetylated lysine, and lysines with other modifications.
Background: Lysine N-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Acetylation of histones can occur at several different lysine positions in the histone tail, and is performed by Histone Acetyltransferases (HATs) such as HAT1 or GCN5. Acetylation of histones is often associated with transcriptional activation. Clone RM101 reacts to lysine-acetylated proteins. No cross reactivity with nonacetylated lysine, and lysines with other modifications.
Product Categories/Family for anti-Acetyl-Lysine antibody
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Product Notes
The Acetyl-Lysine (Catalog #AAA389512) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Acetyl-Lysine antibody (rAb)(Clone RM101) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Acetyl-Lysine can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Western Blot (WB). Applications Validated: WB: 0.5-2ug/ml ICC: 2-10ug/ml. Researchers should empirically determine the suitability of the Acetyl-Lysine for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Acetyl-Lysine, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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