Rabbit TDP43 ELISA Kit | TDP43 elisa kit

Rabbit TDP43 (Tar DNA Binding Protein 43KDa)ELISA Kit

Cat. Num. AAA2501017

• Reactivity: Rabbit
• Synonyms: TDP43; Rabbit TDP43 (Tar DNA Binding Protein 43KDa)ELISA Kit; TDP43 elisa kit

• Reactivity: Rabbit
• Specificity: This kit recognizes natural and recombinant Rabbit TDP43. No significant cross-reactivity or interference between Rabbit TDP43 and analogues was observed.
• Sequence Length: 416

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 3.125-200ng/mL
• Sensitivity: Min: 1.875ng/mL; Max: 200ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TDP43 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rabbit TDP43 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TDP43. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TDP43 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TDP43, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TDP43. You can calculate the concentration of TDP43 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 130750552
• Molecular Weight: 31,808 Da
• NCBI Official Full Name: TDP43
• UniProt Protein Name: TAR DNA-binding protein 43
• UniProt Gene Name: TARDBP
• UniProt Synonym Gene Names: TDP43; TDP-43
• UniProt Entry Name: TADBP_HUMAN

Uniprot Description

TARDBP: DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR. Homodimer. Binds specifically to pyrimidine-rich motifs of TAR DNA and to single stranded TG repeated sequences. Binds to RNA, specifically to UG repeated sequences with a minimun of six contiguous repeats. Interacts with ATNX2; the interaction is RNA-dependent. Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: nucleoplasm; perichromatin fibrils; cytoplasm; nuclear speck; nucleus

Molecular Function: identical protein binding; protein binding; mRNA 3'-UTR binding; RNA binding; double-stranded DNA binding; nucleotide binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; RNA splicing; positive regulation of insulin secretion; positive regulation of transcription from RNA polymerase II promoter; mRNA processing

Disease: Amyotrophic Lateral Sclerosis 10, With Or Without Frontotemporal Dementia