Rabbit LOXL4 ELISA Kit | LOXL4 elisa kit

Rabbit LOXL4 (Lysyl Oxidase Like Protein 4) ELISA Kit

Cat. Num. AAA2504239

• Gene Names: LOXL4; LOXC
• Reactivity: Rabbit
• Synonyms: LOXL4; Rabbit LOXL4 (Lysyl Oxidase Like Protein 4) ELISA Kit; LOXL4 elisa kit

• Reactivity: Rabbit
• Specificity: This kit recognizes natural and recombinant Rabbit LOXL4. No significant cross-reactivity or interference between Rabbit LOXL4 and analogues was observed.
• Sequence Length: 756

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 125-8000pg/mL
• Sensitivity: Min: 75pg/mL; Max: 8000pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LOXL4 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rabbit LOXL4 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to LOXL4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for LOXL4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain LOXL4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of LOXL4. You can calculate the concentration of LOXL4 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 67782349
• NCBI GeneID: 84171
• NCBI Accession #: NP_115587.6
• NCBI GenBank Nucleotide #: NM_032211.6
• UniProt Accession #: Q96JB6; Q5W0B3; Q96DY1; Q96PC0; Q9H6T5
• Molecular Weight: 84,483 Da
• NCBI Official Full Name: lysyl oxidase homolog 4
• NCBI Official Synonym Full Names: lysyl oxidase-like 4
• NCBI Official Symbol: LOXL4
• NCBI Official Synonym Symbols: LOXC
• NCBI Protein Information: lysyl oxidase homolog 4; lysyl oxidase related C; lysyl oxidase-like 4 pseudogene; lysyl oxidase-like protein 4; lysyl oxidase-related protein C
• UniProt Protein Name: Lysyl oxidase homolog 4
• Protein Family: Lysyl oxidase
• UniProt Gene Name: LOXL4
• UniProt Synonym Gene Names: LOXC
• UniProt Entry Name: LOXL4_HUMAN

NCBI Description

This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq, Jul 2008]

Uniprot Description

LOXL4: May modulate the formation of a collagenous extracellular matrix. Belongs to the lysyl oxidase family.

Protein type: EC 1.4.3.-; Secreted, signal peptide; Secreted; Oxidoreductase

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: extracellular space; membrane; receptor complex

Molecular Function: protein binding; copper ion binding; protein-lysine 6-oxidase activity; scavenger receptor activity

Biological Process: receptor-mediated endocytosis