Rabbit anti-Human, Mouse SET7 Polyclonal Antibody | anti-SET7 antibody

SET7, ID (Histone H3-K4 Methyltransferase SETD7, H3-K4-HMTase SETD7, SET Domain-Containing Protein 7, Set9, SET7/9, Lysine N-methyltransferase 7, Histone-lysine N-methyltransferase SETD7, KMT7, KIAA1717) (Azide free) (HRP)

Cat. Num. AAA6499169

• Gene Names: SETD7; KMT7; SET7; SET9; SET7/9
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: SET7; Polyclonal Antibody; ID (Histone H3-K4 Methyltransferase SETD7; H3-K4-HMTase SETD7; SET Domain-Containing Protein 7; Set9; SET7/9; Lysine N-methyltransferase 7; Histone-lysine N-methyltransferase SETD7; KMT7; KIAA1717) (Azide free) (HRP); anti-SET7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SET7. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SET7 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human SET9 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-SET9 Pab is used in Western blot to detect SET9 in mouse brain tissue lysate.)

Western Blot (WB)

(Western blot analysis of SET9 (arrow) using rabbit polyclonal SET9 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SET9 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(SET9 Antibody (S174) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the SET9 antibody detected the SET9 protein (arrow).)

Product Categories/Family for anti-SET7 antibody

Antibodies; Enzymes; Methyltransferase

References

1. Wysocka, J., et al., Genes Dev. 17(7):896-911 (2003). 2. Xiao, B., et al., Nature 421(6923):652-656 (2003). 3. Kwon, T., et al., EMBO J. 22(2):292-303 (2003). 4. Nishioka, K., et al., Genes Dev. 16(4):479-489 (2002). 5. Wilson, J.R., et al., Cell 111(1):105-115 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 83035135
• NCBI GeneID: 80854
• NCBI Accession #: NP_085151.1
• NCBI GenBank Nucleotide #: NM_030648
• UniProt Accession #: Q8WTS6; Q0VAH3; Q4W5A9; Q9C0E6; B5WWL3
• Molecular Weight: 40,721 Da
• NCBI Official Full Name: Homo sapiens SET domain containing (lysine methyltransferase) 7 (SETD7), mRNA
• NCBI Official Synonym Full Names: SET domain containing (lysine methyltransferase) 7
• NCBI Official Symbol: SETD7
• NCBI Official Synonym Symbols: KMT7; SET7; SET9; SET7/9
• NCBI Protein Information: histone-lysine N-methyltransferase SETD7; H3-K4-HMTase SETD7; SET domain-containing protein 7; histone H3-K4 methyltransferase SETD7; histone H3-lysine 4-specific methyltransferase; lysine N-methyltransferase 7
• UniProt Protein Name: Histone-lysine N-methyltransferase SETD7
• Protein Family: SET domain-containing protein
• UniProt Gene Name: SETD7
• UniProt Synonym Gene Names: KIAA1717; KMT7; SET7; SET9; H3-K4-HMTase SETD7
• UniProt Entry Name: SETD7_HUMAN

Uniprot Description

SETD7: Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Interacts with IPF1/PDX-1. Widely expressed. Expressed in pancreatic islets. Belongs to the histone-lysine methyltransferase family. SET7 subfamily.

Protein type: Amino Acid Metabolism - lysine degradation; EC 2.1.1.43; Methyltransferase, protein lysine; Methyltransferase

Chromosomal Location of Human Ortholog: 4q28

Cellular Component: nucleoplasm; nucleolus; chromosome

Molecular Function: protein binding; p53 binding; protein-lysine N-methyltransferase activity; histone-lysine N-methyltransferase activity

Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; regulation of transcription, DNA-dependent; peptidyl-lysine di-methylation; peptidyl-lysine mono-methylation; chromatin modification; response to DNA damage stimulus