Rat ANG I ELISA Kit | ANG I elisa kit
Rat ANG I (Angiotensin I) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ANG I. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat ANG I and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ANG I, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat ANG I. You can calculate the concentration of Rat ANG I in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an exceedingly potent mediator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. In addition, the mature peptide has antimicrobial activity against some bacteria and fungi, including S. pneumoniae and C. albicans. Alternative splicing results in two transcript variants encoding the same protein. This gene and the gene that encodes ribonuclease, RNase A family, 4 share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region. [provided by RefSeq, Aug 2014]
Uniprot Description
ANG: May function as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. Defects in ANG are the cause of susceptibility to amyotrophic lateral sclerosis type 9 (ALS9). ALS is a degenerative disorder of motor neurons in the cortex, brain stem and spinal cord. ALS is characterized by muscular weakness and atrophy. Belongs to the pancreatic ribonuclease family.
Protein type: EC 3.1.27.-; Motility/polarity/chemotaxis; Secreted; Extracellular matrix; Cell cycle regulation; RNA-binding; Nucleolus; Ribonuclease; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 14q11.1-q11.2
Cellular Component: extracellular space; angiogenin-PRI complex; growth cone; cell soma; nucleolus; basal lamina; nucleus
Molecular Function: heparin binding; rRNA binding; protein binding; copper ion binding; DNA binding; endonuclease activity; actin binding; peptide binding; receptor binding; ribonuclease activity
Biological Process: cell migration; negative regulation of smooth muscle cell proliferation; diacylglycerol biosynthetic process; activation of protein kinase B; response to hormone stimulus; activation of phospholipase A2; oocyte maturation; rRNA transcription; positive regulation of protein secretion; ovarian follicle development; homeostatic process; phospholipase C activation; actin filament polymerization; negative regulation of translation; response to hypoxia; positive regulation of endothelial cell proliferation; cell communication; angiogenesis; positive regulation of phosphorylation; placenta development
Disease: Amyotrophic Lateral Sclerosis 9
