Human Lysyl Oxidase Like Protein 4 (LOXL4) ELISA Kit | LOXL4 elisa kit

Human Lysyl Oxidase Like Protein 4 (LOXL4) ELISA Kit

Cat. Num. AAA2019099

• Gene Names: LOXL4; LOXC
• Reactivity: Human
• Synonyms: Lysyl Oxidase Like Protein 4 (LOXL4); Human Lysyl Oxidase Like Protein 4 (LOXL4) ELISA Kit; LOXC; Lysyl oxidase homolog 4; Lysyl oxidase-related protein C; LOXL4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lysyl Oxidase Like Protein 4 (LOXL4).; No significant cross-reactivity or interference between Lysyl Oxidase Like Protein 4 (LOXL4) and analogues was observed.
• Sequence Length: 756

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lysyl Oxidase Like Protein 4 (LOXL4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lysyl Oxidase Like Protein 4 (LOXL4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LOXL4 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysyl Oxidase Like Protein 4 (LOXL4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lysyl Oxidase Like Protein 4 (LOXL4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysyl Oxidase Like Protein 4 (LOXL4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lysyl Oxidase Like Protein 4 (LOXL4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for LOXL4 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 67782349
• NCBI GeneID: 84171
• NCBI Accession #: NP_115587.6
• NCBI GenBank Nucleotide #: NM_032211.6
• UniProt Accession #: Q96JB6; Q5W0B3; Q96DY1; Q96PC0; Q9H6T5
• Molecular Weight: 84,483 Da
• NCBI Official Full Name: lysyl oxidase homolog 4
• NCBI Official Synonym Full Names: lysyl oxidase-like 4
• NCBI Official Symbol: LOXL4
• NCBI Official Synonym Symbols: LOXC
• NCBI Protein Information: lysyl oxidase homolog 4
• UniProt Protein Name: Lysyl oxidase homolog 4
• Protein Family: Lysyl oxidase
• UniProt Gene Name: LOXL4
• UniProt Synonym Gene Names: LOXC
• UniProt Entry Name: LOXL4_HUMAN

NCBI Description

This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq, Jul 2008]

Uniprot Description

LOXL4: May modulate the formation of a collagenous extracellular matrix. Belongs to the lysyl oxidase family.

Protein type: Secreted; Secreted, signal peptide; EC 1.4.3.-; Oxidoreductase

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: extracellular space; membrane; receptor complex

Molecular Function: protein binding; copper ion binding; protein-lysine 6-oxidase activity; scavenger receptor activity

Biological Process: receptor-mediated endocytosis