Testing Data
Rabbit Dimethyl-Histone H3 (Lys27) Antibody | anti-Dimethyl-Histone H3 (Lys27) antibody
Rabbit anti Dimethyl-Histone H3 (Lys 27)
Reactivity
Human, Rat, Mouse, Chicken, Bovine
Applications
ELISA, Western Blot, Chromatin Immunoprecipitation, Immunoprecipitation, Immunohistochemistry
Purity
The Rabbit IgG is purified by Epitope-specific Affinity Chromatography.
Synonyms
Dimethyl-Histone H3 (Lys27); Rabbit anti Dimethyl-Histone H3 (Lys 27); Rabbit anti-Dimethyl-Histone H3 (K27); Histone H3 dimethylated at Lys27 (H3-K27me2); H3K27[Me2].; anti-Dimethyl-Histone H3 (Lys27) antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse, Chicken, Bovine
Specificity
This antibody only recognizes ~ 17 kDa of Dimethylated Histone H3 at K27. It does not cross react to nonmethylated H3 or mono-, Tri-methylated Histone H3.
Purity/Purification
The Rabbit IgG is purified by Epitope-specific Affinity Chromatography.
Form/Format
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
Concentration
Size: 100 ug/200 ul (varies by lot)
Applicable Applications for anti-Dimethyl-Histone H3 (Lys27) antibody
ELISA, Western Blot, ChIP, Immunohistochemistry
Application Notes
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 1:100
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 1:100
Antigen Preparation
A synthetic peptide containing AR[Me2-K]ST in which Me2-K corresponds to Dimethyl lysine 27 of human histone H3.
Positive Control
3T3 Nuclear Extract
Cellular Location
Nuclear
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Testing Data
Testing Data
Related Product Information for anti-Dimethyl-Histone H3 (Lys27) antibody
Histones are basic nuclear proteins that together with DNA make up the nucleosome structure in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The methylation of specific residues in the histone tails is a central modification for regulatingepigenetic transitions in chromatin. Whereas methylation of histone H3 on lysine 4, 36, and 79 has been linked with gene activation, methylation of H3 on lysines 9, 27 and histone H4 on lysine 20 is associated with heterochromatin and some repressed genes with euchromatin. Modified lysine residues can exist in a mono-, di-, or tri-methylated state, while the arginine residues can be mono- or di-methylated. Histone H3 Lys4 trimethylation (H3-K4me3) is a conserved mark of actively transcribed chromatin. This antibody is specific for histone H3 tri-methylated at K4. The sequence is found in all mammals and a wide range of species, including D. melanogaster, Arabidopsis, Chicken and Xenopus. The antibody will react with any of the above species where the trimethylation modification is present.
References
Vakoc C et al. Histone H3 Lysine 9 Methylation and HP1gamma Are Associated with Transcription Elongation through Mammalian Chromatin. Molecular Cell (2003), Volume 19, Issue 3, Pages 381 - 391. David J Seward, et al. Demethylation of trimethylated histone H3 Lys4 in vivo by JARID1 JmjC proteins. Nature Structural & Molecular Biology 14, 240 - 242 (2007)
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Product Notes
The Dimethyl-Histone H3 (Lys27) (Catalog #AAA462179) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Dimethyl-Histone H3 (Lys 27) reacts with Human, Rat, Mouse, Chicken, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's Dimethyl-Histone H3 (Lys27) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, ChIP, Immunohistochemistry. Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml Immunohistochemistry: 1:100. Researchers should empirically determine the suitability of the Dimethyl-Histone H3 (Lys27) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dimethyl-Histone H3 (Lys27), Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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