Rabbit Histone H3, Dimethyl Monoclonal Antibody | anti-H3 antibody

Histone H3, Dimethyl (Lys4)

Cat. Num. AAA600365

• Reactivity: Human, Monkey, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Synonyms: Histone H3; Dimethyl; Monoclonal Antibody; Dimethyl (Lys4); Anti -Histone H3; anti-H3 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 7H27
• Specificity: Detects endogenous levels of histone H3 only when di-methylated on Lys4. Does not crossreact with non-methylated, mono-methylated, or tri-methylated Lys4. In addition, does not crossreact with di-methylated or tri-methylated histone H3 Lys9, Lys27, Lys36 or histone H4 Lys20. Species Crossreactivity: Human, monkey, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol and less than 0.02% sodium azide.

• Applicable Applications for anti-H3 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, ChIP, Immunohistochemistry and Immunocytochemistry.; Dilution: Western Blot: 1:1000; Immunohistochemistry (paraffin): 1:1500; Immunofluorescence (IF-IC): 1:800; Immunoprecipitation: 1:25; Chromatin IP: 1:50
• Immunogen: Synthetic peptide (KLH-coupled) corresponding to the amino terminus of histone H3 in which Lys4 is di-methylated.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of whole cell lysates from HeLa, NIH/3T3, C6 and COS cells using Di-Methyl-Histone H3 (Lys4).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Di-Methyl-Histone H3 (Lys4).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human stomach carcinoma using Di-Methyl-Histone H3 (Lys4).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of HeLa cells using Di-Methyl-Histone H3 (Lys4) (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).)

ELISA (EIA)

(Di-Methyl-Histone H3 (Lys4) specificity was determined by peptide ELISA. The graph depicts the binding of the antibody to pre-coated di-methyl histone H3 (Lys4) peptide in the presence of increasing concentrations of various competitor peptides. As shown, only the di-methyl histone H3 (Lys4) peptide competed away binding of the antibody.)

Related Product Information for anti-H3 antibody

The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination (1). Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (2,3). Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4) (4). In contrast, a more diverse set of histone lysine methyltransferases have been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing (4). Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1) and WD-40 domains (WDR5) (5-8). The recent discovery of histone demethylases such as PADI4, LSD1, JMJD1, JMJD2 and JHDM1 has shown that methylation is a reversible epigenetic mark (9).

Product Categories/Family for anti-H3 antibody

Antibodies; Abs to Histones

NCBI and Uniprot Product Information

• NCBI GI #: 47551065
• NCBI GeneID: 373335
• NCBI Accession #: NP_999709.1
• NCBI GenBank Nucleotide #: NM_214544.1
• NCBI Official Full Name: histone H3
• NCBI Official Symbol: H3
• NCBI Protein Information: histone H3
• Protein Family: Tyrosine phosphatase