Testing Data
Rabbit PAK4 (pS474) Antibody | anti-PAK4 (pS474) antibody
Rabbit anti Phospho-PAK4 (pSer474)
Reactivity
Human, Rat, Mouse, Bovine, Chicken, Canine
Applications
ELISA, Western Blot, Immunoprecipitation
Purity
The Rabbit IgG is purified by phosphospecific-Epitope Affinity Purification.
Synonyms
PAK4 (pS474); Rabbit anti Phospho-PAK4 (pSer474); Rabbit anti-PAK4 (pS474); p21-activated kinase 4; anti-PAK4 (pS474) antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse, Bovine, Chicken, Canine
Specificity
This antibody recognizes the phosphorylated peptide only. It recognizes PAK4 protein at a phosphorylation site of Serine 474 (~68kDa), or serine 602 of PAK5 (~90kDa) or Serine 560 of PAK6 (~80 kDa). It does not cross react to other PAK1, PAK2 or PAK3. This antibody reacts with human, rat and mouse. The other species are not tested.
Purity/Purification
The Rabbit IgG is purified by phosphospecific-Epitope Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer
Concentration
Size: 100 ug/200 ul (varies by lot)
Applicable Applications for anti-PAK4 (pS474) antibody
ELISA, Western Blot, Immunoprecipitation
Application Notes
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Antigen Preparation
A synthetic peptide surrounding the epitope RKSLV-with a phosphorylation at Serine 474 of human PAK4. This sequence is also identical within human, rat, mouse, chicken, bovine and dog species.
Positive Control
HEK293
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Testing Data
Testing Data
Related Product Information for anti-PAK4 (pS474) antibody
p21-activated protein kinase (PAK) serine/threonine kinases are important effectors of Rho family GTPases and have been implicated in the regulation of cell morphology and motility, as well as in cell transformation. The phosphorylation of serine 474 as the likely autophosphorylation site in the kinase domain of PAK4 occurs in vivo. PAK4 is frequently over-expressed in human tumor cell lines of various tissue origins, indicates that the phosphorylation of PAK4 is required in oncogenic transformation and Ras-driven, anchorage-independent growth.
References
Marinella G. Callow et al. Requirement for PAK4 in the Anchorage-independent Growth of Human Cancer Cell Lines. (2002) J. Biol. Chem. 277 (1), 550-558.
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Product Notes
The PAK4 (pS474) (Catalog #AAA462102) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Phospho-PAK4 (pSer474) reacts with Human, Rat, Mouse, Bovine, Chicken, Canine and may cross-react with other species as described in the data sheet. AAA Biotech's PAK4 (pS474) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunoprecipitation. Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml. Researchers should empirically determine the suitability of the PAK4 (pS474) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAK4 (pS474), Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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